Microbial interactions for 2,4,6-Trinitrotoluene biotransformation /

(Bartha, 1990). Pure culture approach to biodegradation of

Bibliographic Details
Main Author: Sivaraju, Muruganandam
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1996.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:(Bartha, 1990). Pure culture approach to biodegradation of
2amDNT and 4amDNT, with the p-nitro group being more
2species > isolates. The growth, measured as optical density,
advantages and disadvantages. Pure culture studies allow a
Alcaligenes sp. Five of these isolates, Ent. sp. 15, Pseu.
and mixed cultures in the aerobic biotransfonnation of TNT.
as an isolate. It appears that faster transformation of TNT
bacterial species, isolated from a munitions waste
capabilities, and enhance the significance of genetic
compounds, higher quantitative and qualitative biodegradative
contaminated site in Illinois, were tentatively identified as
exchange between different species leading to the evolution
existing in environments containing xenobiotic compounds,
for the complete biodegradation of certain xenobiotics, since
for this study to investigate the system efficiency of pure
function as a community. Microbial "teamwork" is required
has been directed towards pure culture studies with very
in the following order: 5-species > 4-species > 3-species >
intermediates, and products, coupled with simplicity and
is observed in mixed cultures as compared to pure cultures.
little emphasis on mixed populations. Researchers have
mixed populations have a higher resistance to toxic
more detailed evaluation of pathways, enzymes, degradation
Much of the research in the past on the biodegradation of TNT
observed that, on many occasions, microorganisms capable of
of novel degradative activities (Bull, 1980). Nine aerobic
one Enterobacter sp., one Pseudomonas sp., and seven
preferentially reduced over the o-nitro group.
reactors containing Pseu. sp. 17 in mixed cultures, but not
reproducibility for bioremediation of xenobiotics. However,
Sorption/desorption processes were observed in certain
sp. 17, and three Alc. sp. I 1 3, 11 5 and 122 were selected
species. The average percentages of TNT transformations were
than that of any single component species of that community
the average sum of the transformations of the component
The isolates were mixed in various combinations of doublets,
the metabolic diversity of a microbial community is greater
TNT transformation were higher for the mixed cultures than
to the isolates. The major intermediates identified were
triplets, quadruplets, and quintuplet. The percentages of
was more pronounced in the mixed culture reactors as compared
xenobiotic and natural recalcitrant products have numerous
Item Description:"Major subject: Civil Engineering".
In title, numerals are used.
Vita.
Physical Description:xi, 149 leaves : illustrations ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.