Development and characterization of a bovine interspecific hybrid backcross panel : linkage analysis, gene mapping, and cytogenetics /
A panel of 40 Bos taurus x Bos gaurus (gaur) interspecific
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| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=743274041&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A panel of 40 Bos taurus x Bos gaurus (gaur) interspecific hybrid backcross offspring was constructed to be used as a tool for refining and improving the resolution of the bovine gene map. of the bovine gene map. The utility of interspecific backcrosses has already been proven in the development of the mouse gene map, as a result of increased probability of parental heterozygosity at a given marker. The gaur (2n=58) is characterized karyotypically is characterized karyotypically by a 2;28 Robertsonian translocation with respect to the cattle karyotype (2n=60). This translocation was used as a centromeric marker to directionally orient linkage maps of Bos taurus chromosomes BTA 2 and BTA 2 8. Markers tested in the linkage analysis represented genes or DNA segments previously placed on bovine maps, as well as markers chosen from bovine-humanmouse comparative maps. Additionally, fibroblast cultures from fetal tissue of each offspring were analyzed cytogenetically at early passages. In all cases, the translocation chromosome was inherited in a balanced condition, and only one cell line possessed a chromosome abnormality. The trisomic line was karyotyped as 61,XXX. Among the DNA markers analyzed, MYF6, SKMC, COX4, COX6AH, COX6AL, COX8H, COX8L, ALPI, CHAT, DBI, and SRM represent additions to the bovine gene map. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xiii, 77 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |