In vitro co-culture of bovine embryos using Buffalo Rat Liver Cells, CR1aa and TMC199 /
(FCS), bovine serum albumin fraction V (BS"), or FCS + BS",
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (FCS), bovine serum albumin fraction V (BS"), or FCS + BS", 3 70 C in a humidified atmosphere of 5% C02 in air. After 7 an additional 48 h to determine embryo viability by hatching. blastocyst stage was compared between different treatment BRLC (P<.05) or in TCM199 with BRLC (P=.0632). No embryos Cells (BRLC) in CRlaa, without BRLC in CRlaa or with BRLC in cryopreservation, than did those cultured in CRlaa without cryopreservation. These results indicate that embryos can be cultured in a defined medium; however, no embryos cultured in cultured in CRlaa without BRLC hatched after days, the number of oocytes developing to the CM and/or Embryonic development was enhanced when FCS or FCS + BSAV was embryos cultured in CRlaa alone or with BRLC in TCM199. Experiment I were cryopresevered, thawed and co-cultured for groups. Embryos were co-cultured with Buffalo Rat Liver groups. More embryos cultured in CRlaa with BRLC developed In Experiment 2, compact morulae and blastocysts from More embryos cultured in CRlaa with BRLC survived potential 1-cell embryos were divided into 12 treatment respectively. In Experiment 1, the embryos were cultured at Seventeen hundred sixty-one in vitro produced bovine stage in CRlaa with BRLC than in CRlaa without BRLC (P<=.OS). supplemented with polyvinyl alcohol (PVA), fetal calf serum the absence of BRLC survived cryopreservation. Tissue Culture Medium 199 (TCM 1 99). The media were to the CM or blastocyst stage (P<.OS) when compared to used in conjunction with CRlaa with BRLC, compared to TCM199 with BRLC., More embryos developed to the CM or blastocyst |
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| Item Description: | "Major subject: Veterinary Physiology". In title, numerals are used. Vita. |
| Physical Description: | ix, 60 leaves ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |