Stable nitrogen isotope measurements of marine bacterial proteins and nucleic acids : tracers of microbial activity /
([]1.0%[]) heavier than the whole cells, confirming previously
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | ([]1.0%[]) heavier than the whole cells, confirming previously ) heavier than nucleic acids. Protein and nucleic acid []15N reliably reflects whole cell []15N. In batch culture 515N were determined to reflect whole cell values. Estimates accurately reflected the proportions of these two allochthonous Mississippi River input. also averaged 3%[] to 4%[] (+1.0%[]) during exponential and associated with nutrient uptake and assimilation are bacterial protein extracts, varied as a function of growth bacterial proteins and nucleic acids from cultures and calculated for whole cells, marine and estuarine samples were clearly distinguished, as were waters influenced by environmental samples were analyzed to determine if protein established procedure averaged 1.6%[] lighter than cells. experiments, the net fractionation between Vibrio harveyi and extracts from estuarine and marine water samples were extracts that averaged 0.8%[] heavier than whole cells. in aquatic ecosystems if the enzyme mediated fractionations intracellular nitrogen pools. Both protein and nucleic acid large volume cultures with proteins being []2.8 [] 0.8%[] (n=l0 lntracellular discrimination in natural samples averaged less Mass balance of protein and nucleic acid accurately reflected measured and bacterial population []15N values were estimated. negligible or consistently predictable. In this study, NH4+, however, exhibited more variable discrimination but nitrogen assimilation. Organic nitrogen assimilation Nucleic acid extracts prepared using a separate, previously of intracellular discrimination were strongly linear for rate and substrate. Exponential cell growth resulted in a reduction of discrimination during both organic and inorganic reported values. Protein extracts from cultures assimilating resulted in protein extracts that averaged 3%[] to 4% Stable nitrogen isotopes ([]15N) can trace elemental cycling stationary growth. Large volume batch culture reduced the suspended particulate material (SPM), was compared with that than half that observed in culture (n=10). When the []15N of the than cells. Mass balance of protein and nucleic acid variation in measured discrimination and resulted in protein |
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| Item Description: | "Major subject: Oceanography". Vita. |
| Physical Description: | xi, 73 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |