Brucella abortus : analysis of bovine monocytes and macrophages stimulated with soluble and particulate Brucella antigens /
A 62 kDa brucellae protein that shares more than 60% amino acid similarity with members of GroEL heat shock protein (Hsp) family has been described. After challenge with live Brucella abortus, peripheral blood mononuclear cells from 49% of the animals responded to Hsp62. One-day-old, tissue cultur...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=743273481&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A 62 kDa brucellae protein that shares more than 60% amino acid similarity with members of GroEL heat shock protein (Hsp) family has been described. After challenge with live Brucella abortus, peripheral blood mononuclear cells from 49% of the animals responded to Hsp62. One-day-old, tissue culture-derived monocytes were better antigen presenting cells (APC) of Hsp62 than 7 day old tissue culture-derived macrophages. . The cell population responding to brucella Hsp62 was identified as CD4+ T cells. The T cell response to three cloned deletion Hsp62 mutants (N and C terminus deletions) demonstrated an epitope(s) between amino acid residues 365-494 and 435-494. Human T-cell clones respond to mycobacterial Hsp65 epitopes found between wnino acid residues 100-300 and 451-465. 'Me brucella Hsp62 does not appear to be an immunodominant antigen. Bovine CD4+ T cells respond to distinct epitopes located in the C-terminus of Hsp62. Since our previous experiments demonstrated a difference in the APC capacity between bovine monocytes and macrophages, these cells were stimulated with one of the following: y-irradiated B. abortus S2308, brucella cytosol proteins from strain RB5 1, or live B. abortus S2308. Following stimulation, cells were harvested and tested for expression of cell surface markers and cytokine genes. Expression of MHC class H molecules (DP, DQ, DR) on macrophages, unstimulated and imulated, were decreased as compared to the monocytes. However, stimulation with bruceflae antigens did not alter expression of MHC class 11 molecules, CD 1 8, or Mae- I on either monocytes or macrophages. Gene expression of IL- I P, IL-4, IL-6, IL- IO, IL- 12, and TNF-(X using reverse transcription 77 RNA polymerase-dependent amplification was determined. Expression of either IL-1p, IL-4, or IL-6 was increased in macrophages stimulated with live B. abortus S2308, but there was no consistent pattern among different animals. In all animals, little or no IL-10 or IL-12 was expressed and the level of TNF-cc was similar regardless of the stimulus. The expression of these cell surface molecules or cytokine genes do not appear to affect the APC capacity of monocytes versus macrophages, since no consistent differences were demonstrated among different animals. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". |
| Physical Description: | x, 83 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |