rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Necrotizing hepatopancreatitis in Penaeus vannamei
    sub title: molecular phylogeny and detection of the etiologic agent and pathogenesis in an experimental model of the disease
  name:
    name part: Loy, James Kenneth
    name part: 1962-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1994
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiii, 130 leaves : illustrations ; 28 cm.
  abstract: Necrotizing hepatopancreatitis (NHP) is a severe disease of
  abstract: farm-raised Pacific white shrimp (Penaeus vannamei) that has
  abstract: been associated with mortalaties approaching 95%. The
  abstract: etiology of NHP is a pleomorphic, Gram-negative bacterium.
  abstract: The NHP bacterium has not been cultivated in vitro and little
  abstract: is known about the life cycle of the NHP bacterium outside
  abstract: the shrimp host epithelial cell. In this study, we used the
  abstract: polymerase chain reaction (PCR) to amplify the gene that
  abstract: encodes the 16S ribosomal RNA (16S RDNA) of the NHP
  abstract: bacterium. The amplified 16S RDNA was then cloned and
  abstract: sequenced. Phylogenetic analysis of the sequenced 16S RDNA
  abstract: enabled placement of NHP bacterium in the (X-Proteobacteria.
  abstract: As phylogenetic categories are predictive, the information
  abstract: that is known about one organism can generally be applied to
  abstract: its relatives. Using information derived from the
  abstract: phylogenetic classification.of the NHP bacterium, potential
  abstract: reservoir hosts and environmental sources of the organism are
  abstract: currently being investigated. In addition, the 16S RDNA
  abstract: sequence was used to develop a sensitive and specific PCR
  abstract: detection assay and a NHP bacterium-specific 16S RDNA probe.
  abstract: In this study, the PCR assay was used to confirm NHP in
  abstract: shrimp cultured in Texas and Peru. The DNA probe was tested
  abstract: in an in situ hybridization assay and was shown to be
  abstract: efficacious for early detection of the NHP bacterium in
  abstract: infected shrimp. Using electron microscopy and in situ
  abstract: hybridization, the temporal pathogenesis of NUP was
  abstract: investigated. In this study, we demonstrated that the
  abstract: initial infection most likely occurs through endocytosis of
  abstract: the NHP bacterium by the epithelial cell and the NHP
  abstract: bacterium is spread to adjacent hepatopancreatic epithelial
  abstract: cells through lateral transmission. In this study, we
  abstract: demonstrated that the NHP bacterium can be degraded by the
  abstract: shrimp epithelial cell, provided that the organism is
  abstract: confined to the phagosome. If, however, the NHP bacterium
  abstract: escapes from the phagosome it can replicate within the
  abstract: cytoplasm to reach the massive numbers seen in the natural
  abstract: infections. These findings have important implicafions in
  abstract: the efficacious treatment of NHP.
  note: by James Kenneth Loy.
  note: Vita.
  note: "Major Subject: Veterinary Pathology".
  note: Issued also on microfiche from University Microfilms Inc.
  note: Includes bibliographical references.
  note: Ph. D. Texas A&M University 1994.
  subject:
    topic: Major veterinary pathology
  identifier: 96-34883 UMI
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 970303
    record change date: 20190325093240.0
    record identifier: in00001334511