Tissue culture based systems to validate the enzymatic detoxification of organophosphorus neurotoxicants /
3) alterations in ionophore-induced Ca 21 fluxes in
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | 3) alterations in ionophore-induced Ca 21 fluxes in addressing different aspects of OP toxicity is indicated for amelioration by organophosphate hydrolase (OPH) were-. 1) assays utilized for detecting OP neurotoxicity and its attenuate inhibition of enzyme systems, such detoxification by mipafox and 56.5% by hydrolyzed mipafox) suggesting that cells and from 2% to 86% in SY5Y cells. OPH did not suppress compounds, paraoxon had no effect of Ca'+ fluxes in cells contrary to expectations based on effects of other OP detoxification of organophosphorus (OP) neurotoxicants. The diethyl phosphonic acid. Also cells treated with hydrolyzed exposed to ionomycin. Cells treated with hydrolyzed paraoxon fluorescence units) and OPH alone (871 fluorescence units) inhibition of acetylcholine esterase (AChE), 2) inhibition of ionomycin (1 195 fluorescence units) than cells treated with It was observed that OPH suppressed the ability of paraoxon may augment effects on [Ca 2+] i due to the production of mipafox hydrolysis proceeds at a slow rate. Microfluoroscopy neurotoxic esterase (NTE), and NTE inhibition by mipafox within 5 minutes (59.5% inhibition organic phosphonic acids. Thus a battery of assays paraoxon (874 fluorescence units), p-nitrophenol (951 paraoxon and p-nitrophenol showed significantly different recovery rates. The results of this study suggest that showed a higher final fluorescence level after recovery from suggesting a potential role for paraoxon breakdown product SY5Y (human neuroblastoma) and PC12 (rat pheochromocytoma). systems to test the efficacy of enzyme prophylaxsis for the This study was conducted to develop cell culture based though enzymatic detoxification of OPs may selectively to inhibit ACHE, restoring activity from 33% to lOO% in PC12 transformed neuronal cell lines validating enzymatic detoxification of OPs. with the fluorescent calcium probe fluo-3 suggested that, |
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| Item Description: | "Major subject: Toxicology". Vita. |
| Physical Description: | x, 64 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |