http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
model for equine breed identification using microsatellites and posterior probability testing
(E. caballus) and from an Arabian half-sib family. PCR
0.5426 and 0.5295 were observed in Arabians and
allelic diversity with an average of 4.8947 alleles per locus
amplification was also attempted for homologous loci in
an average Fst 0. 1 126 over all loci. Although each
analyzed by polyacrylantide gel electrophoresis followed by
and 3.4737 alleles per locus and an average heterozygosity of
and an average heterozygosity of 0.5642. An average of 4.0526
and Ddm. The draft-origin breed group showed the greatest
Arabians and Thoroughbreds. The model utilized a posterior
autoradiography. Segregation analysis of the 19 n-
based on the microsatellite allele distributions observed in
between any of the loci. Microsatellite allele distributions
classified, 20.59% were indistinguishable and 2.94% were
considerably with the distance measure that was used. Ds
distance using three different distance estimates, Ds, Dps
distances than any intra-specific comparison under the same
either pure Arabian (ARAB), a pure Thoroughbred (THOR) or a
estimates were greatest (0.480 to 2.050). Genetic distance
estimates were intermediate (0.334 to 0.476) and Ddm
estimates were most conservative (0. 130 to 0.268), Dps
Fifteen new horse Microsatellite loci were identified by
first generation ARAB-THOR hybrid. Thirty-four test horse
for calculation of population differentiation and genetic
generate the relative likelihood that an individual was
genetic distance measure reflected similar cladistic
genotypes were analyzed and of these, 76.47% were correctly
Grant's zebra, E. burchelli granti. PCR products were
granti. Each distance measure showed greater horse-zebra
in the unrelated breed groups of E. caballus were compared
measure. A model for horse breed classification was designed
measures were also applied to pairwise comparisons between
microsatellite loci did not indicate linkage (Z > 3.0)
performed on DNA from three breed groups of unrelated horses
primer pairs complementary to flanking region DNA were
probability algorithm across a multi-locus genotype to
probe. Positive clones were sequenced and oligonucleotide
relationships, the magnitude of divergence varied
screening a size-fractionated plasrnid library with a (GT) 15
synthesized. Polymerase chain reaction (PCR) assays were
the breeds of horses tested ranged from 0.0029 to 0.4171 with
the individual breed groups of E caballus and E burchelli
Thoroughbreds, respectively. Genetic differentiation among
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