A model for equine breed identification using microsatellites and posterior probability testing /
(E. caballus) and from an Arabian half-sib family. PCR
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (E. caballus) and from an Arabian half-sib family. PCR 0.5426 and 0.5295 were observed in Arabians and allelic diversity with an average of 4.8947 alleles per locus amplification was also attempted for homologous loci in an average Fst 0. 1 126 over all loci. Although each analyzed by polyacrylantide gel electrophoresis followed by and 3.4737 alleles per locus and an average heterozygosity of and an average heterozygosity of 0.5642. An average of 4.0526 and Ddm. The draft-origin breed group showed the greatest Arabians and Thoroughbreds. The model utilized a posterior autoradiography. Segregation analysis of the 19 n- based on the microsatellite allele distributions observed in between any of the loci. Microsatellite allele distributions classified, 20.59% were indistinguishable and 2.94% were considerably with the distance measure that was used. Ds distance using three different distance estimates, Ds, Dps distances than any intra-specific comparison under the same either pure Arabian (ARAB), a pure Thoroughbred (THOR) or a estimates were greatest (0.480 to 2.050). Genetic distance estimates were intermediate (0.334 to 0.476) and Ddm estimates were most conservative (0. 130 to 0.268), Dps Fifteen new horse Microsatellite loci were identified by first generation ARAB-THOR hybrid. Thirty-four test horse for calculation of population differentiation and genetic generate the relative likelihood that an individual was genetic distance measure reflected similar cladistic genotypes were analyzed and of these, 76.47% were correctly Grant's zebra, E. burchelli granti. PCR products were granti. Each distance measure showed greater horse-zebra in the unrelated breed groups of E. caballus were compared incorrectly classified. measure. A model for horse breed classification was designed measures were also applied to pairwise comparisons between microsatellite loci did not indicate linkage (Z > 3.0) performed on DNA from three breed groups of unrelated horses primer pairs complementary to flanking region DNA were probability algorithm across a multi-locus genotype to probe. Positive clones were sequenced and oligonucleotide relationships, the magnitude of divergence varied screening a size-fractionated plasrnid library with a (GT) 15 synthesized. Polymerase chain reaction (PCR) assays were the breeds of horses tested ranged from 0.0029 to 0.4171 with the individual breed groups of E caballus and E burchelli Thoroughbreds, respectively. Genetic differentiation among |
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| Item Description: | "Major subject: Genetics". Vita. |
| Physical Description: | xi, 94 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |