Gene expression in physically impeded maize roots /
a relatively conserved gene family exists in maize. The CDNA
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1996.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | a relatively conserved gene family exists in maize. The CDNA about 33% by 10 to 30 min physical impedance, is identical to acid residues which showed 97% similarity at the nucleic acid Arabidopsis, and LP2 induced by drought stress in pine, had characterized further. cDNA PIIGI contains 678 hp with an expression was strongly induced by the 10 min physical extracted from stressed and non-stressed maize roots tips. found duplicated loci in maize but single loci in rice. The from mMRNA from a 10 min physical impedance treatment of function. Genomic Southern hybridization using cDNApIIG2 high homology with the RNA in maize root tips, but they did hybridized to mRNA from maize root tips. The results showed impedance treatment and genomic Southern analysis showed that induced in a 10 min physical impedance treatment, were level to maize root cortical cell delineating protein. maize roots tips and was differentially screened with maize roots tips. Second, a cDNA library was constructed Northern analysis with cDNA PIIGI as a probe showed that the not reveal an inducible pattern of expression in the impeded open reading frame which specifies a polypeptide of 129 amino physical impedance. First, cDNA clones induced by mechanical pIIG2 has a nucleotide sequence of 830 bp with an open radioactive labeled cDNA probes synthesized using mRNA reading frame which specifies a polypeptide of 210 amino acid residues, but in a search of the GENBANK database it did not responding to a 10 min physical impedance stress. The first search. show significant homology with any identified gene of known stress or drought stress of other plant species were that only two clones, TCH1 induced by wind stress in the partial sequence of maize "calreticulin!' gene by GENBANK third cDNA clone pIRG3, 800 bp, whose expression was reduced Three clones, PIIGI, pIIG2, and pIRG3, were identified as Two approaches were used to search for genes which respond to two cDNA clones (PIIGI and pIIG2), whose expressions were |
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| Item Description: | "Major subject: Plant Physiology". Vita. |
| Physical Description: | x, 86 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |