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mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Analysis of cyclin-dependent kinases as sensitive indicators of cellular proliferation in teleost embryos and larvae
  name:
    name part: Westerman, Mark Edward
    name part: 1954-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1995
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xvi, 135 leaves : illustrations ; 28 cm.
  abstract: Morphological, biochemical, and molecular studies of cellular
  abstract: proliferation in developing teleost embryos and larvae were
  abstract: performed. Temperature-dependent embryonic developmental
  abstract: rates of geographically distinct populations of Fundulus
  abstract: heteroclitus were determined in the laboratory by
  abstract: morphological staging. Embryos from Massachusetts
  abstract: populations developed faster and hatched earlier than embryos
  abstract: of Florida populations at all temperatures examined, and had
  abstract: lower incipient lethal temperature limits. Results suggest
  abstract: intrinsic embryonic developmental rates between populations
  abstract: differ during cleavage stage and that this adaptation results
  abstract: in appropriate hatching during the spring tidal cycle.
  abstract: Analysis of pl3suc1-precipitated proteins from F.
  abstract: heteroclitus embryos, demonstrated proteins similar or
  abstract: identical to the cyclin-dependent kinase (cdk), p34cdc2, and
  abstract: cyclin B. Appropriately sized, pl3suc1-precipitated proteins
  abstract: crossreacted with antiPSTAIR and cyclin B antibodies, readily
  abstract: phosphorylated histone HI protein and peptide, and had
  abstract: oscillating activity relative to cleavage cell cycles
  abstract: consistent with identification as MPF. p34suc1-precipitated
  abstract: MPF did not contain phosphotyrosine over the first three cell
  abstract: cycles following fertilization and had a Km for ATP and
  abstract: histone HI peptide of 51.83 and 11.97 uM, respectively. A
  abstract: quantitative assay of cdk enzyme activity was developed and
  abstract: cdk kinetic parameters defined for Sciaenops ocellatus. Suc
  abstract: 1-precipitated cdks from S. ocellatus had a Km for ATP and
  abstract: histone H1 peptide of 97.34 and 13.65 JIM, respectively.
  abstract: Reaction conditions stabilized cdks and allowed linear
  abstract: incorporation of labeled phosphate into a histone H I peptide
  abstract: over a two hour period. Temperature and feeding level
  abstract: effects on cdk activity in developing S. ocellatus larvae
  abstract: were determined to examine an hypothesis that cdk activity
  abstract: may accurately reflect cellular proliferation in teleost
  abstract: embryos and larvae. Results of two growth experiments
  abstract: clearly demonstrated that in both experiments, tissues of
  abstract: rapidly growing larvae had significantly higher MPF activity
  abstract: and MPF larva-I than more slowly growing cohorts on 5 of 6
  abstract: days analyzed. MPF activity larva-I had a large dynamic
  abstract: range, scaled with larval growth rates, and may reflect
  abstract: developmental-proliferative events. Results support analysis
  abstract: of cdk activity as a sensitive and cell cycle specific
  abstract: indicator of cellular proliferation in teleost embryos and
  abstract: larvae.
  note: by Mark Edward Westerman.
  note: Vita.
  note: "Major Subject: Wildlife and Fisheries Sciences".
  note: Issued also on microfiche from University Microfilms Inc.
  note: Includes bibliographical references.
  note: Ph. D. Texas A&M University 1995.
  subject:
    topic: Major wildlife and fisheries sciences
  identifier: 96-15920 UMI
  identifier: http://hdl.handle.net/1969.1/DISSERTATIONS-1576542
  identifier: http://proxy.library.tamu.edu/login
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    location:
      url: http://hdl.handle.net/1969.1/DISSERTATIONS-1576542
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    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 961015
    record change date: 20210803090658.0
    record identifier: in00001323933