Studies on a PDV envelope protein of Autographa californica nuclear polyhedrosis virus /
A baculovirus late gene encoding a 66 kDa structural protein (PDV-E66) of Autographa californica multinucleocapsid nuclear polyhedrosis virus (ACMNPV) has been mapped in the viral genome, cloned, sequenced, and its expression in infected cells studied. Transcription of PDVE66 initiates from two con...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=739663331&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A baculovirus late gene encoding a 66 kDa structural protein (PDV-E66) of Autographa californica multinucleocapsid nuclear polyhedrosis virus (ACMNPV) has been mapped in the viral genome, cloned, sequenced, and its expression in infected cells studied. Transcription of PDVE66 initiates from two conserved upstream TAAG motifs. PDV-E66 protein is present from 24 h p.i. in AcMNPV-infected cells as detected by an antibody specific to PDV-E66. PDVE66 is an integral membrane protein of the PDV envelope as determined by Western-blot analysis of fractionated viral envelopes and nucleocapsids, Triton X-114 assay, and immunogold labeling of purified PDV. In the infected cell nucleus, PDV-E66 is present in the intranuclear viral-induced microvesicles and PDV envelope, providing more direct evidence that the microvesicles may function as an immediate precursor in the assembly of the PDV envelope during PDV envelope nuclear maturation. Our studies also focus on the signals and pathways for the transport and assembly of membrane proteins in the nucleus. A N-terminal region of 23 amino acids, which is composed predominantly of hydrophobic residues, was shown to be necessary and sufficient to target a cytoplasmic marker protein, P-galactosidase, to the intranuclear viral-induced microvesicies and viral envelope during viral infection. This amino acid sequence also inserts P-galactosidase into microsomal membranes in the rabbit reticulocyte in vitro translation system. This is the first report of an amino acid sequence which can specifically target a foreign protein to the intranuclear microvesicles and PDV envelope. This sequence will be utilized to further study the pathways and origin(s) of the viral-induced intranuclear membrane structures. Possible topology of PDV-E66 in the membrane and pathways of PDV-E66 nuclear targeting are discussed. |
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| Item Description: | Vita. "Major Subject: Biology". |
| Physical Description: | xii, 231 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |