Hyperbaric oxygen and hypoxia alter production of nitric oxide by J774 murine macrophages /
bacterial clearance may partly stem from changes in
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | bacterial clearance may partly stem from changes in clearance in infected tissue during intermittent exposure to comparing HBO-treated cells that were incubated with concentration and as the percent change of nitrite concentration from control (no HBO), within each experiment. control cells, ([], respectively). Cells incubated in 957o Control cells, incubated in P02 20mmHg or 40mmHg, produced determine if hypoxia serves concomitantly with other factors determining concentrations of nitrite in cellular did cells exposed to 95% room air (RA), 5% C02- Cells different oxygen tensions; however, cells that were incubated exposed to HBO (12 PSIg), significantly (P < 0.0001) hyperbaric oxygen (HBO) have been documented. Increased Improvements in rates of healing of wounds and bacterial in wounds while exposed to increased oxygen tensions that increased production of NO when compared to their untreated incubated in P02 = 20mmHg or 40mmHg, and intermittently incubated with 95% RA. These data show that, 1) J774 murine interferon (y-INF) and lipopolysaccharide (LPS). Production intermittently exposed to HBO. Further research will M[] exposed to relatively low oxygen tensions, produce less NO than do those which are incubated in 95% room air, and NO, 13.5 ︢9.2%, than did HBO-treated cells that were occur during HBO treatments. This theory was tested using of nitric oxide was assayed spectrophotometrically by of NO, (1 1.4 ︢11.47o), when compared to control cells. production of nitric oxide (NO) by resident macrophages (M(D) RA and treated with HBO showed little increase in production significantly (P < 0.0001) less NO, ([] respectively), than supernatant. Results were expressed as [LM nitrite that, 2) production of NO is augmented when these cells are the murine M[] cell line J774, stimulated with gamma- therapy may alter production of NO by M[] in wounds. There was no significant difference in production of NO when to increase production of NO in this model. Intermittent HBO with P02 = 20mmHg and treated with HBO produced slightly more |
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| Item Description: | "Major subject: Veterinary Physiology". In title, numerals are used. Vita. |
| Physical Description: | x, 38 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |