Evaluation of alternative methodologies for the detection of Salmonella enteritidis in table eggs /
(4 of 36). There is no evidence from these experiments to
| Main Author: | |
|---|---|
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
|
| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (4 of 36). There is no evidence from these experiments to albumen pools (centrifugation technique), a sample from each albumen was pooled, treated with papain and N-Acetyl-L- an additional five experiments, Single Comb White Leghorn and chemical reduction of albumen pools to allow for and incubated at room temperature for 3 d prior to direct at selected times post-challenge and cultured for SE using between the room temperature technique (6 of 35) and the PCR brilliant green agar (BGA). In three of the experiments, centrifugation and detection of SE by culture and the centrifugation technique and the PCR, yolk and albumen from concentration of Salmonella enteritidis (SE) by cysteine, centrifuged, and the resulting pellet incubated in detection of SE in eggs derived from experimentally infected evaluation for SE by the PCR using primers specific for five or six eggs in each group were pooled, thoroughly mixed, following concentration, centrifugation, and enrichment of hens were orally challenged with SE, and eggs were collected hens, while providing presumptive identification of SE 72 h However, the centrifugation technique and the PCR allowed for identification of SE than the room temperature protocol. In is comparably sensitive to room temperature culture for the members of the genus Salmonella. For comparison to the more rapid (48 h or 72 h respectively) presumptive not observed in the number of SE positive pools detected plating on BGA. Results indicated the three techniques were polymerase chain reaction (PCR). In six experiments, eggs pool was subjected to DNA extraction and subsequent samples as positive. These experiments suggest that the PCR SE/pool to .6 cfu SE/pool) in experimentally inoculated eggs. similar in sensitivity for detection of SE (range: 72 cfu sooner. suggest that the PCR will erroneously identify negative tetrathionate broth at 37 C for 24 h prior to plating on The following studies describe the use of enzymatic digestion the three culture techniques. Significant differences were were inoculated with low numbers of viable SE. Albumen and yolk from five or six eggs in each group were separated, the |
|---|---|
| Item Description: | "Major subject: Poultry Science". Vita. |
| Physical Description: | x, 44 leaves ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |