DNA structure(s) recognized and bound by large subunit of Replication Factor C (ls RFC) in Drosophila melanogaster /
(Southwestern) assays; however, it is bound approximately
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (Southwestern) assays; however, it is bound approximately 1) used DNase and micrococcal nuclease footprinting assays to Alternative (non-B type) DNA structures are thought to be angles or bends in the DNA and may recognize the replication better than single stranded DNA or duplex DNA. This suggests bound by lsRFC in nitrocellulose filter binding bound by lsRFC. Osmium tetroxide chemical modification of chemical probing using osmium tetroxide was performed and complex binding. A synthetic primer-template junction is define a primer template junction as a substrate for the RFC determinant(s) in the DNA structure. lsRFC is an essential DNA polymerase delta onto the replication template during five fold less efficiently than branched DNA structures but fork formed at the junction between parental and daughter fork. formed as intermediates in developmentally important important role in the regulation of cellular mechanisms. mechanisms such as DNA replication, DNA repair, and genetic of eukaryotic DNA replication. RFC complex loads PCNA and oligonucleotides containing internal complementary sequences part of the five subunit RFC complex involved in the process predicted branched structures. Tsurimoto and Stillman (I 99 present in the branched DNA structures are most strongly proposed that lsRFC preferentially recognizes and binds recognized by lsRFC. To investigate the determinant present, recombination. It is proposed that these alternative DNA replication and is required both for leading and lagging Replication Factor C) is a protein that binds to the DNA in a reveals either single strand or distorted duplex nature at sequence independent manner and recognizes structural Southwestern assays and the chemical modification data, it is strand synthesis. Southwestern analyses using synthetic strands. This proposed intimate association of lsRFC with structural aspects of the DNA. lsRFC (large subunit structure(s) and the proteins that recognize them may play an that bind to the DNA by recognizing specific sequence and/or that HP96 contains a determinant that is preferentially the proposed primer template junction. Based on the the replication fork may influence the progression of the There has been an enhancement in the knowledge of proteins these synthetic DNA structures verifies the formation of the to form DNA structures reveal that structural determinants |
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| Item Description: | "Major subject: Biology". Vita. |
| Physical Description: | x, 50 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |