Characterization of endosperm proteins in quality protein maize /
Maize endosperm proteins were studied to observe their
| Main Author: | |
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=742535521&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Maize endosperm proteins were studied to observe their concentration changes during seed development and their relation to endosperm lysine contents and endosperm hardness. Albumins and globulins (35, 38, 43, 92, and 100 kDa) of normal and o2 M017 endosperm decreased during maturity. Glutelins of similar molecular weights increased concurrently during maturity. These observations suggest that the albumin and globulin proteins are crosslinked by disulfide bonds to form the glutelin protein matrix in endosperm. Zein content increased during maturation of normal maize endosperm. Zein synthesis in o2 endosperm ends after 25 days after pollination (DAP), except for the 19 kDa (x-zein. This results in lower zein contents in o2 than in normal endosperm. Glutelins of normal maize endosperm increased up to 35 DAP, while they continued to increase until maturity in o2 maize. Therefore, glutelin contents were lower in mature normal than in mature o2 endosperm. Glutelins contribute more lysine in QPM endosperm than other proteins due to their amounts and lysine contents (average 5.4%). Zeins were negatively correlated to endosperm lysine contents of both normal and o2 maize. Six glutelins were significantly correlated to endosperm lysine content and significantly correlated to each other. Thus, glutelins could be good candidates for molecular markers for the selection of QPM. The 19 and 22 kDa cc-zeins and 27 kDa y-zein were significantly correlated to kernel density. Anti zeins and 92 kDa glutelin antisera specifically cross reacted with their target proteins on western blots. Intensities obtained from Spot-Blot ELISA of 22 kDa (x-zein, 14 kDa O-zein, 35 and 84 kDa glutelins significantly correlated to corresponding intensities obtained from SDS-PAGE. Lysine contents of twenty nine inbreds significantly correlated to intensities of anti 35 kDa protein obtained using Spot-Blot. Endosperm hardness of twenty nine inbreds were significantly correlated to anti 22 kDa (x-zein. Increasing lysine contents and endosperm hardness are important in the QPM breeding program. Using quantitative immunoassay has the potential to facilitate QPM breeding program. |
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| Item Description: | Vita. "Major Subject: Food Science and Technology". |
| Physical Description: | xvi, 118 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |