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| LEADER |
00000ctm a22000005a 4500 |
| 001 |
in00001315768 |
| 005 |
20190325093131.0 |
| 008 |
960627s1994 xx a b 000 0 eng d |
| 035 |
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|a (OCoLC)34999699
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| 035 |
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|9 AHB0147AM
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| 037 |
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|a 95-20450
|b UMI
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| 040 |
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|a TXA
|c TXA
|d UtOrBLW
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| 049 |
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|a TXAM
|a TXAR
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| 099 |
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|a 1994
|a Dissertation
|a P311
|
| 100 |
1 |
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|a Patnaik, Ranjan,
|d 1969-
|
| 245 |
1 |
0 |
|a Overexpression of pyruvate kinases and phosphoenolpyruvate synthase in Escherichia coli :
|b physiological characterization and application /
|c by Ranjan Patnaik.
|
| 264 |
|
1 |
|a [Place of publication not identified] :
|b [publisher not identified] ;
|c 1994.
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| 300 |
|
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|a xiv, 91 leaves :
|b illustrations ;
|c 28 cm.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
|
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|a unmediated
|b n
|2 rdamedia
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| 338 |
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|a volume
|b nc
|2 rdacarrier
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| 504 |
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|a Includes bibliographical references.
|
| 500 |
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|a Vita.
|
| 502 |
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|b Ph. D.
|c Texas A&M University
|d 1994.
|
| 500 |
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|a "Major Subject: Chemical Engineering".
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| 530 |
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|a Issued also on microfiche from University Microfilms Inc.
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| 520 |
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|a Escherichia coli (E. coli) is perhaps the most widely studied
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| 520 |
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|a prokaryote by both scientists and engineers in the area of
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| 520 |
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|a biological sciences. On one hand scientists are trying to
|
| 520 |
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|a unravel and understand the biochemical and genetic regulation
|
| 520 |
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|a of various metabolic pathways as has evolved in the bacterial
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| 520 |
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|a cell, while on the other hand engineers are constantly on the
|
| 520 |
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|a look out for constructing and designing new strains by
|
| 520 |
|
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|a altering existing pathways for improving production of
|
| 520 |
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|a chemicals. In most cases these engineered strains are quite
|
| 520 |
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|a different from the wild type. Engineers use the insights
|
| 520 |
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|a from the paradigms developed by the scientists to narrow down
|
| 520 |
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|a their choices for manipulating strains for a specific
|
| 520 |
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|a purpose, and the characterization of this engineered strain
|
| 520 |
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|a serves as feedback in the understanding of that paradigm,
|
| 520 |
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|a either strengthening it or invalidating it. In this study a
|
| 520 |
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|a combined attempt is made in which alterations in the central
|
| 520 |
|
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|a metabolism of E. coli are characterized from a fundamental
|
| 520 |
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|a perspective and then a new central metabolism is created that
|
| 520 |
|
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|a improves production of 3-deoxy-D-arabino-heptulosonate7-
|
| 520 |
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|a phosphate (DAHP) over an existing industrial strain. The
|
| 520 |
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|a central metabolism is altered by overexpression of a
|
| 520 |
|
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|a gluconeogenic enzyme, phosphoenolpyruvate synthetase (Pps)
|
| 520 |
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|a under glycolytic conditions and also by overexpression of
|
| 520 |
|
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|a pyruvate kinases (PykI, Pykll). These three enzymes along
|
| 520 |
|
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|a with the glucose phosphotransferase system (PTS) contribute
|
| 520 |
|
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|a significantly in the modulation of the intracellular levels
|
| 520 |
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|a of two key metabolites of the central metabolism,
|
| 520 |
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|a phosphoenolpyruvate (PEP) and pyruvate. Insights obtained
|
| 520 |
|
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|a from this study were successfully used in directing more
|
| 520 |
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|a carbon flux from PEP towards aromatics production. The
|
| 520 |
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|a following results were obtained: (1) Overexpression of PykH
|
| 520 |
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|a observable effect on the specific growth rate and the glucose
|
| 520 |
|
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|a consumption rate in E. coli. However, overexpression of Pps
|
| 520 |
|
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|a increased glucose uptake rate, oxygen consumption rate,
|
| 520 |
|
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|a fermentation product excretion, and showed activity dependent
|
| 520 |
|
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|a growth inhibition. (2) Overexpression of Pps in combination
|
| 520 |
|
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|a with DAHP synthase (AroG) and transketolase (TktA) increases
|
| 520 |
|
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|a the yield of DAHP production from glucose by almost twofold
|
| 520 |
|
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|a over the control strains to near theoretical levels.
|
| 650 |
|
4 |
|a Major chemical engineering.
|
| 856 |
4 |
1 |
|u http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=741965761&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD
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|
| 952 |
f |
f |
|p noncirc
|a Texas A&M University
|b College Station
|c Cushing Memorial Library & Archives
|s cush tdr
|d Cushing: Theses & Disserertations (Remote Storage: 2-3 day retrieval)
|t 0
|e 1994 Dissertation P311
|h Other scheme
|i unmediated -- volume
|
| 952 |
f |
f |
|a Texas A&M University
|b College Station
|c Electronic Resources
|s www_evans
|d Available Online
|t 0
|e 1994 Dissertation P311
|h Other scheme
|
| 998 |
f |
f |
|a 1994 Dissertation P311
|t 0
|l Available Online
|
| 998 |
f |
f |
|a 1994 Dissertation P311
|t 0
|l Cushing: Theses & Disserertations (Remote Storage: 2-3 day retrieval)
|