Effects of nitrogen source and concentration upon glutamine synthetase and protease activity in the rumen bacterium Prevotella ruminicola strain B1 4 /
12.5 mM respectively, were used for the non-limited group.
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | 12.5 mM respectively, were used for the non-limited group. A series of experiments was conducted to determine the and effective bacterial protease inhibitors. and sodium carbonate) with variable amounts of ammonium appeared to be positively correlated with the previously are used as the sole source of nitrogen P. ruminicola becomes assays. Nitrogen concentrations of 15.0 mM, 15.0 mM, and basal medium (mineral salts, essential volatile fatty acids, be influenced by nitrogen concentration such that activity cells grown in either nitrogenlimited or non-limited chloride (18.715 units) and casei n (1 4.42 u n its), and all chloride, pepticase or casein as the nitrogen source. The concentrations of 1.0, 1 0.0, and 50.0 m no growth was conditions. No activity was observed in the non-limited culture becomes nitrogen limited at 2.5 mM. These three effects of varying nitrogen sources and concentrations upon Experiments with MSX, a potent inhibitor of glutamine Glutamine synthetase activity was measured from mid-log phase glutamine synthetase and protease activities in Prevotella growth. The protease activity assays indicated that had the highest activity (20.755 units) followed by ammonium However, when casein is the sole source of nitrogen the increases when nitrogen availability decreases. This may limited groups for the glutamine synthetase and protease nitrogen concentrations were then used as the nitrogen nitrogen limited when nitrogen concentration is at 0.5 mM. nitrogen-limited cultures had higher proteolytic activity non-limited conditions MSX had no significant effect upon observed glutamine synthetase activities. The results of observed under nitrogen-limiting conditions. However, in the of growth studies showed that when MSX was used in proteases that could facilitate the development of practical provide some insight into the regulation of rumen bacterial results indicated that when ammonium chloride or pepticase ruminicola strain B,4. P. ruminicola was grown on a defined synthetase during nitrogen-limited conditions. The results synthetase, indicated the relative importance of glutamine than non-limited cultures. Moreover, these activities this study indicate that protease activity in the rumen may treatments were significantly different (p < 0. 05). treatments. However, in the limited treatments, pepticase vitamin cofactors, 12 mM glucose, cysteine-HCL as a reductant |
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| Item Description: | "Major subject: Nutrition". In title, numerals are used. Vita. |
| Physical Description: | x, 60 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |