Isolation and characterization of Babesia caballi specific DNA probe and B. caballi recombinant proteins from a genomic DNA library in Lambda Zapii /
Babesia caballi DNA from infected erythrocytes was used to
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| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=741966061&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Babesia caballi DNA from infected erythrocytes was used to construct a genomic library in the expression vector lambda ZAPII. Four clones expressing the same or closely related recombinant protein were identified. The recombinant clones contain a B.caballi insert DNA of 1.5 kDa -1.6 k]Da as confirmed by Southern blot hybridization with biotin labeled,B. caballi DNA. Monospecific antibody to the recombinant protein made by antibody selection from hyperimmune serum reacted with B. caballi antigen in IFAT and in Western blot. The recombinant protein of approximately 55 kDa is an antigenic homologue of the 37 kDa B. caballi antigen. The DNA insert of one of the clones Bcl, was further characterized to develop a B. caballi specific DNA probe. The Bcl based DNA probe hybridized to 7 ng of B. caballi DNA but not to 3 mg of DNA from B. ecrui, B. bovis, B. biaemina, Ana-plasma marqinale and horse white blood cells. The B, caballi specific DNA probe identified gl infected blood sample with 35% Restriction blood cells. The B, caballi specific DNA probe identified B.caballi DNA from a 12.5 gl infected blood sample with 35% Pcv and 0.31% parasitemia. Restriction enzyme experiments and Southern blot analysis suggested that BC1 insert at least 2 times represented in the B.Caballi genome. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". |
| Physical Description: | xiv, 87 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |