Cryopreservation of equine oocytes using glycerol as the cryoprotectant and cumulus investment as a parameter /
A study was conducted to determine the viability of equine
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| Format: | Thesis eBook |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | A study was conducted to determine the viability of equine abattoir and transported to the lab in physiologic saline and 3) corona radiata only. All oocytes were stained with bound organelles such as endoplasmic reticulum and Golgi, categories based on cumulus investment. These categories cryopreserved equine oocytes could not be determined. establishing they were nonviable. The oocytes were then expansion as a second check of viability. None of the fluorescein diacetate (FDA) to determine viability before freezing. Forty viable oocytes from each group were frozen frozen equine oocytes as compared to fresh equine oocytes loss of microvilli on oocyte, disruption of single membrane maturation media. The oocytes were then stained with orcein membrane. The protocol evaluated in this study was not metaphase 11 after 36 h in the maturation media, further methods in this experiment. migration of intact organelles away from the periphery of the needle. Recovered oocytes were assigned to one of three of the 120 oocytes showed fluorescent activity, thus One viable oocyte from each group was frozen to be evaluated oocyte and lack of cortical granule alignment at oocyte cell oocytes cryopreserved in glycerol and to determine whether oocytes exhibited cumulus expansion after exposure to the placed in maturation media and evaluated visually for cumulus postthaw by transmission electron microscopy. The most significant ultrastructural differences observed in the solution. Follicles were aspirated using a syringe and stage of cumulus investment at freezing had an effect on successful in maintaining viability of equine oocytes their survival. Ovaries were collected from mares at an Therefore, the effect of cumulus morphology on viability of throughout the freeze-thaw process as evaluated by the to be evaluated post-thaw using FDA and orcein stains. None to determine metaphase state. None of the oocytes were in verifying that none of the oocytes were viable post-thaw. were disrupted plasma membranes of cumulus cells and oocyte, were: 1) complete cumulus layer; 2) partial cumulus layer; |
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| Item Description: | "Major subject: Physiology of Reproduction". Vita. |
| Physical Description: | xi, 65 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |