A genetic study of mycelial compatibility groups of Sclerotium rolfsii /
"Universal Primers" from ribosomal DNA. When amplification
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | "Universal Primers" from ribosomal DNA. When amplification amplified by polymerase chain reaction (PCR) using the arbitrarily- collected from plants showing symptoms of based on the presence or absence of an antagonism zone (a clearing of mycelia) between paired colonies. These isolates consistently yielded the same restriction pattern and several could be placed in one of 12 MCG. MCG 6 was detected most frequently and was identified among isolates obtained from grouped according to mycelial compatibility; 262 isolates identified in isolates collected from other locations in MCG shared a common pattern. When a 18-base oligonucleotide patterns were observed. Again, all isolates within a MCG had patterns were observed. All isolates within an MCG primer, NK2, was used in PCR, three distinct amplification products of approximately 685 base pairs were digested with RDNA from several isolates belonging to different MCG was restriction endonuclease Mbo 1, four restriction digest Sclerotium rolfsii isolates from Texas peanut fields were southern blight in four central Texas fields, and hyphal Texas. The internal transcribed spacer (ITS) region of the the same pattern and several MCG shared a common pattern. three widely separated fields. An additional seven MCG were tipped, were examined for mycelial compatibility groups (MCG) |
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| Item Description: | "Major subject: Plant Pathology". Vita. |
| Physical Description: | xi, 63 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |