Purification and characterization of a 50 kDa glycoprotein in cercariae of Schistosoma mansoni /
(2-D) and 2-D western analyses revealed that gp5O is a
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (2-D) and 2-D western analyses revealed that gp5O is a (gp5O) was found to be unique to ConA-purified cercarial A affinity chromatography. A dominant 50 kDa glycoprotein Antigenicity study using infected mouse sera showed that gp5O antigens (ConA-Cer), but absent in ConA-SEA or ConA- SWAP. body, indicating that it may function during the penetration. butalso with prepatent 4-week serum. Furthermore.. theN- carbohydrates of gp5O belong to complex type N-linked communes lectins. Using confocal laser scanning microscopy, discuss its use as a potential prepatent immunodiagnostic glucose; GIcNAc and/or sialic acid; galactose and/or GaINAc. Glycoproteins of S. mansoni were purified using concanavalin glycosylation with many accessible sugars: mannose and/or gp5O by infected mouse sera. Two dimensional electrophoresis gp5O was localized to the penetration glands of the cercarial gp5O was tested as a diagnostic marker using prepatent and haematobium and F. hepatica, but not S. japonicum. Finally, Investigations on the expression of gp5O within different life cycle stages showed that gp5O polyclonal antibody (PAb) linked oligosaccharides are required for the recognition of not only reacted with 8- and 16-week infected mousesera, of gp5O and its strong reaction with prepatent human sera, we on the nature of gp5O glycosylation demonstrated that the One of the interesting results is that gp5O is the only patent human patient sera. Because of the unique properties protein in ConA-Cer which can be recognized by Ricinus react with eggs. On the other hand, gp5O PAb showed cross- reacted with a 60 kDa protein in adult worms, but did not reactivity with other trematode parasites, including S. reagent towards schistomiasis and as a vaccine candidate. single protein with an isoelectric point (pl) of 8. Studies |
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| Item Description: | "Major subject: Veterinary Microbiology". In title, numerals are used. Vita. |
| Physical Description: | x, 109 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |