Histone H1 proteins in Chlamydomonas reinhardtii /
(SDS-PAGE) of 5% perchloric acid (PCA) extracts of isolated
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (SDS-PAGE) of 5% perchloric acid (PCA) extracts of isolated acid-urea gels. Digestion of the algal Hl proteins with V8 along with an H2B protein which was partially extracted by and Hlb relative to each other when Triton was added to the by two-dimensional electrophoresis, and analyzed by peptide C. reinhardtii nuclei revealed two Hl proteins (Hia and Hlb) cells. This analysis suggested that the ratio decreases in databases revealed no sequence homology of the Hla N-terminus dimensional gels of 5% PCA extracts of pea leaf nuclei gametes and vegetative cell types were studied by inducing gametes. gametogenesis in C. reinhardtli cultures and analyzing heterogeneity of either algal Hl protein, while two- Hlb/Hla ratios in gametes relative to nongamete control in the hydrophobic amino acid content of the two C. mapping and N-terminal sequencing. C. reinhardtli histones obtained from C. reinhardtii Hla amino terminal sequencing, peptide maps, but peptide maps of algal and pea H2B did show protease revealed two distinctly different peptide maps. C protein spots. Two-dimensional gels did detect differences reinhardtii Hls as evidenced by changing mobilities of Hla reinhardtii were extracted from isolated nuclei, fractionated reinhardtil Hl peptide maps were not similar to pea Hl separated the three Hl bands seen in SDS-PAGE, into five Sodium dodecyl sulfate polyacrylamide gel electrophoresis some peptides in common. Seventeen amino acid residues were the acid. Two-dimensional gel analysis did not reveal The Hl proteins of the unicellular green alga Chlamydomonas were compared to histones collected from pea leaf nuclei. while the Hlb N-terminus was blocked. A search of protein with any known proteins. Changes in HI levels between |
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| Item Description: | "Major subject: Genetics". In title, numerals are used. Vita. |
| Physical Description: | viii, 48 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |