Structural and functional alterations associated with transformation in rat ovarian cell model systems /

a step-wise fashion in the SIGC -> SV-SIGC -> T-SV-SIGC

Bibliographic Details
Main Author:	Echols, Jana Elizabeth
Format:	Thesis eBook
Language:	English
Published:	[Place of publication not identified] : [publisher not identified] ; 1995.
Subjects:	Major veterinary anatomy.
Online Access:	Link to OAKTrust copy

Description
Summary:	a step-wise fashion in the SIGC -> SV-SIGC -> T-SV-SIGC additional cell line (T-SVSIGC). Utilizing indirect Additionally, SIGC were continuously passaged in culture. anchorage-independent, as shown by soft agar assay. The and also showed decreased desmosome expression when compared calcium medium showed a dramatic reduction of desmoglein carcinogenesis. Spontaneously immortalized rat granuloma cells (SIGC) were transfected with either the pSV3neo plasmid clones in soft agar and showed a decrease in desmosomal and cytokeratin associated protein organization. They also cytokeratin pattern density was diminished in the neu-SIGC dramatic decrease compared to SIGC. SIGC subject to low exhibited a decrease in the rate of GJIC and an increase in glutathione levels compared to low passage (23) SIGC. immunofluorescence and monoclonal antibodies, the expression In vitro cell model systems derived from rat granuloma cells junctional intercellular communication (GJIC) was reduced in lower when compared to SIGC, but T-SV-SIGC showed the most mice and the resulting tumors were expanded into an migration in the wound healing assay. Additionally, gap neu-SIGC compared to SIGC. Late passage SIGC readily formed of desmosomes and associated cytokeratins in SV-SIGC was plasmid containing the Her-21neu oncogene (neu-SIGC). staining at cell-cell borders, as well as, single cell structural and functional alterations associated with the system. SIGC transfected with the neu oncogene were The three cell model systems were evaluated according to to SIGC. GJIC was also significantly reduced in the neu-SIGC transformed phenotype. SV-SIGC cells were injected into nude were developed for the study of multistep ovarian when compared to the parental line SIGC. GSH was elevated in which contains the SV40 early region genes (SV-SIGC) or the
Item Description:	"Major subject: Veterinary Anatomy". Vita.
Physical Description:	viii, 72 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics.
Bibliography:	Includes bibliographical references.