Isolation and characterization of five ozone-inductible cDNA clones from Atriplax canescens (saltbush) /
(0112A-3) and the protease (018-3); second, ozone stress may
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (0112A-3) and the protease (018-3); second, ozone stress may 018-3 protein is acids of both clones are glycine-rich proteins, and they have and 0.45 kb) of 1.4 kb species. 0112A-3 showed an increase in are a naked species (about 1.45 kb) and are subfragments (1.0 been observed with the induction of several stress-related change the cell wall components by increasing the amount of Clone 012-1 has strong homology with small subunit RRNA. course of induction and different stresses such as water- damage to increase the expression of the proteinase inhibitor days after ozone fumigation, and rise steadily in response to deficit and sulfur dioxide stress. The encoded proteins deficit stress, and are late-induced by ozone stress. The derived from five different polya RNAs which accumulate in development and produces visible signs of damage. Within a effects to relieve cellular stress. encodes a polypeptide which has homology with wound-inducible examined, and those cDNAs were sequenced. Clone 012-1, few hours of exposure, certain biochemical alterations have glycine-rich proteins (OID2-2 and OIS14-3); and third, ozone homologous to thiol proteases. Clone OID2-2 and OIS14-3 show hybridized with three RNA species, is initially induced in mediated cross-linking, which may use oxyradical-scavenging Northern blots suggest that transcripts hybridized to 012-1 occurs with ozone, sulfur dioxide and water deficit were of ozone-inducible genes varies with respect to the time Ozone fumigation to Atriplex canescens (saltbush) caused the ozone-stressed shrub leaves were isolated by differential plant cells respond to ozone stress by increasing Prolonged exposure of plants to ozone inhibits growth and protein inhibitors. MRNA species corresponding to 018-3 are proteins. accumulation of several polya RNAS. CDNA clones repeated motifs, (G-G-G-Y-G-H)n, and putative cell wall- repressed to prolonged ozone and sulfur dioxide exposure. response to both ozone and water deficit stress, but is response to two pollutants, but not to water deficit. 0112A-3 screening methods. Alteration in gene expression which similar patterns in gene expression Transcript levels of the stress may change the cell wall structure with tyrosine- suggest that: first, ozone stress may generate wounding targeting signal peptides. Northern analyses indicate that transcription of several genes. Furthermore, the expression transiently induced in response to sulfur dioxide and water two genes rise rapidly and remain at a high level by seven water deficit and sulfur dioxide stress. The deduced amino |
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| Item Description: | "Major subject: Plant Physiology". Vita. |
| Physical Description: | xii, 105 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |