Effect of dimethylsulphoxide on fertilization of Bovine oocytes matured in vitro /
05). Normal fertilization rates in the IO and 15 min
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | 05). Normal fertilization rates in the IO and 15 min 5, 10, 15, 20, or 30 min exposure to 1.5M DMSO in Dulbecco's compared to the 20 or 30 min times and to the 20 min control. controls or each other. In the second experiment, oocytes cryoprotectant, DMSO, at room temperature and at O C. In the exposure times were not significantly different from the exposure times. Normal fertilization rate was found to be fertilization rates were found to be significantly different first experiment, oocytes were randomly assigned to either a from controls in the 5, 20 and 3 0 min exposure times (P < 0. in vitro matured bovine oocytes to the addition of the No results were found to support the theory that exposing oocytes to cryoprotectant at O'C rather than 27'C lessens the phosphate-buffered saline at room temperature (27 C). Normal significantly higher in the 15 min exposure to DMSO when The objective of this research was to test the sensitivity of toxicity of the cryoprotectant. were exposed to 1.5M DMSO at 0 C for either 15, 20, or 30 min |
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| Item Description: | "Major subject: Physiology of Reproduction". Vita. |
| Physical Description: | vii, 45 leaves ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |