Typing Mycobacterium bovis isolates from Texas and Mexico using molecular markers /

an insight on the number of different strains prevalent in

Bibliographic Details
Main Author: Perumaalla, Veera Shankar, 1970-
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1995.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:an insight on the number of different strains prevalent in
Analyzer program and a database representing all RFLP types
are more diverse than those from Mexico. Dendrograms based
combination with DR probe. On the whole 95 M. bovis isolates
differentiated into 18 different RFLP types when used in
DR and PGRS. Eighteen different fingerprint types were
exhibited twenty-seven different DR types. By combining the
exhibiting identical RFLP types were found in animals within
fingerprints demonstrate that M. bovis isolates from Texas
fingerprints were used to further differentiate these
generated using the IS6110 probe. But the majority (82%) 71
herds. Fingerprint patterns generated using PGRS probe were
Image analysis was performed using BioImage Whole Band
Importation of infected cattle from Mexico, immigration of
IS6110 type) from Texas and Mexico are genetically closely
isolates. For example, the above isolates (71) were
multiple IS6110 copies in 12 different patterns. DR
of 87 isolates contained a single IS6110 copy distributed on
of Mycobacterium bovis is necessary to characterize potential
of the disease. This study is performed to determine the
on the 'DR'fingerprints shows M. bovis isolates (identical
one of the four different restriction fragments. In an
originating from herds in different geographic regions of
originating from Texas and Mexico. Eighty-eight isolates
outbreaks.
patterns generated by IS6110 and DR probes it was possible to
potential infected farm workers, increased deer farming and
related. The databank of different RFLP types will provide
separate the isolates into 32 distinct RFLP types. Isolates
sources of exposure and the mode of transmission.
Texas and Mexico were analyzed using molecular probes IS6110,
the same herd as well as in animals in geographically distant
this region and improve epidemiological tracking during
too crowded to interpret the differences among the isolates.
transmission between herds are some of the suspected causes
unusual manner, the remaining isolates (18@o) contained
value of molecular markers in typing M. bovis isolates
was established. Dendrograms based on relatedness of IS6110
With the rise of bovine tuberculosis in Texas, strain typing
Item Description:"Major subject: Veterinary Microbiology".
Vita.
Physical Description:x, 64 leaves : illustrations ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.