Isolation and identification of Triticum aestivum cv. Chinese Spring-Aegilops variabilis disomic chromosome addition lines using morphological and molecular markers /
In order to further investigate the genetics of Aegilops
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| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=742145751&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | In order to further investigate the genetics of Aegilops variabilis and the evolutionary relationships between its chromosomes and thoseof hexaploid wheat, a number of T. aestivum cv. Chinese Spring-Ae. variabilis disomic chromosome addition lines have been developed. Morphological and molecular markers, mainly restriction-fragment-length polymorphisms (RFLPs), were used to identify the addition lines. Morphological markers were examined first, followed by molecular markers. In all, six morphological markers, two enzyme structural gene loci, Aco-Uvl andAdk-Svl, and 38 RFLPs markers were localized in Ae. variabilis chromosomes. Thirteen of the 14 possible addition lines have been tentatively identified. Evidence from several sources indicates that each of the 13 lines contains an intact pair of Ae. variabilis chromosomes. The relationships of Ae. variabili,v chromosomes in the addition lines to chromosomes in the ancestral S and U genomes and the A, B, and D genomes of hexaploid wheat have been tentatively determined. The chromosomes contained in addition lines C-1, G-1, K-2, K-1, J-1, N-1, and 1-2 are designated ]Sv, 2Sv, 3Sv, 4Sv, 5Sv, 6Sv, and 7Sv, respectively, and the chromosomes in addition lines A-1, L-1, F-1, H2, L-2, and D-1 are designated lUv, 2Uv, 3Uv, 4Uv, 5Uv, and 6Uv, respectively. The available evidence indicates that only the chromosome 7Uv addition line has not been isolated. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xv, 87 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |