Isolation and characterization of species-specific repetitive DNA sequences of G. longicalyx relative to G. hirsutum /

8 G-. clones were confirmed specific by secondary screens.

Bibliographic Details
Main Author: Landrum, Charles Perry, 1966-
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1994.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:8 G-. clones were confirmed specific by secondary screens.
80 G. sturtianum were selected based on results of the
barbadense may be promoted. Furthermore, probes exhibiting
characterized by hybridization togenomic DNAs of three
chromosomes plus a specific G-. turtianum chromosome. The
Cl-C and Cl-D plants. Lanes of both the Cl-C and Cl-D
cultivated tetraploid species, G. hirsutum and G.
cytogenetic markers, the genetic improvement of the
discriminating among taxonomic groups, and for analyzing
DNA sequences as species-specific molecular and molecular
each of which includes a full complement of G.irsutum
examined were specific for G. turtianum, but none of the II
G.turtianum repetitive DNA clone pCL8 was present in the
genomic DNAs of 16 species representing 7 genomic groups of
hirsutum and G. sturtianum or G. longicalyx genomic DNA.
initial screening. Each of the 4 G. sturtianum and 13 G.
loncjicalyx recombinant plasmids selected in the initial
longicalyx but lacking in G. hirsutum. By using repeated
monosomic addition plants, MA Cl-A, MA Cl-C, and MA Cl-D,
monosomic alien addition
phylogeny. Initially, the species-specific nature of G.
plants showed faint hybridizations relative to the G.
plants tested.
repetitive DNA clone pCL21 was present in all three of the
repetitive DNA sequences present in G. sturtianum and G.
screening were further characterized by hybridization to
species-specificity could provide convenient markers for
sturtianum and G. longicalyx repetitive sequence clones
The G-. turtianum recombinant plasmids were further
the genus Gossypium. Two of the 4 G.turtianum clones
The purpose of this work was to isolate and characterize
Thirteen of 118 G. longicalyx recombinant plasmids and 4 of
turtianum and pentaploid lanes. The G. turtianum
were used in hybridizations to Southern blots of G.
Item Description:"Major subject: Plant Breeding".
Vita.
Physical Description:xii, 52 leaves : illustrations ; 28 cm.
Also available online.
Bibliography:Includes bibliographical references.