Molecular selection : processing genetic information by applying selection to populations of loci represented by DNA molecules /
Most problems in molecular genetics reduce to searching for
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Online Access: | Link to OAKTrust copy http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=742145641&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Most problems in molecular genetics reduce to searching for similarities or differences among individuals. Traditionally, the genome is viewed through the marker paradigm: the state of loci is inferred from genotypes at linked markers located at fixed points throughout the genome. Molecular selection (MS) involves a paradigm shift: the genome is treated as a population of loci denoted a genetic information set (GIS) which is represented by short DNA fragments. Selection is applied to reduce the GIS to those loci that have desired similarities or differences with other GISs. A selection algorithm consists of a series of steps in which a set of GISs are compared using the following operations: subtraction identifies loci that are different, intersection identifies loci that are similar and union pools the loci in multiple GISs. A molecular algorithm is based on a selection algorithm but reflects the characteristics of the applicable molecular operations. MS is applied to mapping utilizing an algorithm that generates a GIS containing only loci from regions concordant with the target trait. An identity by descent (IBD) map is produced by selecting GISs to contain regions in which an individual is IBD with its paternal grandsire(dam) and its maternal grandsire(dam). Inferences apply to regions rather than points. The detection system is based on hybridization to a set of GISs that represent ordered genomic segments, which partition the genome based on recombination events. Probability distributions of the information content available for mapping simply inherited traits from various combinations of phenotypically dominant and recessive full sibs are derived. When complete IBD maps are available, the transmission information content of a resource family can be expressed in terms of the initial phase and relative order of crossovers for each chromosome. Processing genetic information in the form of DNA molecules is more efficient than converting genetic information into analog (visual) representations and converting this to a digital representation for computer processing. The examples pertain to gene mapping, but MS is broadly applicable to most, if not all, areas of genetics. A model system for MS based on microsatellite loci is described and demonstrated in the laboratory. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | x, 110 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilms Inc. |
| Bibliography: | Includes bibliographical references. |