Agrobacterium-mediated transformation of Arachis hypogaea L. utilizing the shoot apex method /
abnormal chromosome counts in greater than 40 % of cells.
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | abnormal chromosome counts in greater than 40 % of cells. accomplished by use of an enzyme hydrolysis technique which All 250 putative transformants exposed to EHA105 were tested amenable to counting. The chromosome numbers for 5 in vivo analyzed by Southern hybridization. The blot showed a faint and analyzed for transient expression of the foreign gene. any areas hybridized by the probe. Somatic chromosome band in both undigested and digested putative transformant by ELISA for presence of the N coat protein, and one-half containing 0.5 mg/L PPT in addition to clavamox. Explants determined. One of the micropropagation derived progeny had EHA101 survived selection on PPT and rooted. These plants explant was transferred to rooting medium containing MS and I genome harbored different foreign gene construct. EHA105 had glucuronidase. Neither test identified putative growth of the explant. Two Agrobacterium tumefaciens strains in some instances, induced by opines. Explants exposed to initiation medium containing MS salts and 0. I mg/L BAP for 5 mg/L NAA. Media having 250 mg/L antibiotic did not inhibit or more weeks. After the shoot elongated to 2 mm, the preparations of root tips of Arachis hypogaea L. (2n=40) were produced slides with hundreds of cells that had chromosomes progeny and 5 in vitro micropropagated progeny seedlings were provided resistance to the herbicide bialaphos. Shoot apices red and PAT spectrophotometric assays. One plant was Regeneration of peanut (Arachis hypogaea L.) plants from the samples. Neither undigested nor digested control plant had selectable marker genes. Bar gene harbored by EHA101 shoot apex of in vitro germinated seedlings was accomplished that survived the co-cultivation were rooted, grown in soil the bar gene were placed on shoot initiation medium the N coat protein gene of tomato spotted wilt virus and two transformants. Six explants that were co-cultivated with used for insertion of foreign genes into the peanut plant were exposed to bacterial culture of one of the constructs, were tested by GUS histochemical assay for insertion of 0- were tested for presence of the bar gene by the chlorophenol with initiation and rooting media. Explants were left on |
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| Item Description: | "Major subject: Plant Physiology". Vita. |
| Physical Description: | xvi, 105 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |