Molecular characterization of AsgA, an unusual signal transduction protein required for multicellular development in Myxococcus xanthus /
AsgA displays acid liability and base stability which is
| Main Author: | |
|---|---|
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1995.
|
| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | AsgA displays acid liability and base stability which is asga gene is one of three known genes required for A-signal asga that contains a kanamycin resistance gene (Kmr) inserted asgA476 allele have similar phenotypes with respect to at various positions within or near asga indicate that asga Cells containing either the Kmr insertion within asga or the characteristic of phosphoramidates such as phosphohistidines. demonstrate that AsgA protein has autokinase activity, and density signal required during early development. We have determined the DNA sequence of asga. The deduced amino acid development of the wild type strain but is absent in an asga development, colony color, and expression of an asg-dependent disruption strain. Phosphorylation assays with purified AsgA domain of AsgA has been found in vitro. We propose that AsgA early development. We have overexpressed asga in E. coli and functions within a signal transduction pathway that is fusion confirms that asga is expressed during growth and gene. An analysis of expression of a translational asgA-lacZ histidine protein kinase domain to the response regulator hydrophobic residues in the N-ten-ninal receiver domain. immunoblotting with the AsgA antibody detected a 42 kD is required for fruiting, but is not essential for growth. members of the histidine protein kinase family and the No evidence for transfer of the phosphoryl group from the production in Myxococcus xanthus. The A-signal is a cell production of extracellular A-signal. proline substitution within a conserved stretch of protein corresponding to the predicted size of AsgA. This protein is present during both vegetative growth and purified AsgA protein. Purified AsgA was used as antigen to raise polyclonal antibodies in rabbits. Western reaction is dependent on the presence of divalent cations regulatory systems. Gene replacement studies using a copy of required to sense starvation and to respond with the response regulator family of the so-called two-component sequence of AsgA exhibits a remarkable similarity to those of such as Mn2+ or Mg2+. The phospholinkage in phosphorylated the autophosphorylation of AsgA occurs very rapidly. This The mutation within asgA476 was found to cause a leucine-to- |
|---|---|
| Item Description: | "Major subject: Biology". Vita. |
| Physical Description: | viii, 67 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references. |