http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Biodegration of hypertrehalosemic hormone in the cockroach Blaberus discoidalis
and Phe4. This cleavage site was indicative of an
and Ser5. it is possible that more than one enzyme might be
Blaberus discoidalis hypertrehalosemic hormone (HTH=
breakdown products of HTH in hemolymph samples and in vitro
concentration of the other two metabolic products indicating
degradation product appeared to be a cleavage between Phe4
degradation was also analyzed by matrix-assisted laser
desorption and ionization mass spectrometry (MALDI-MS).
dYVNFSPGWGT-NH2), an analog of HTH, was cleaved between Asn3
endopeptidase 24.11-like cleavage, but was not inhibited by
incubation medium. Examination of [3H-pQl]HTH by HPLC
indicated that the breakdown products of HTH relative to the
involved in HTH degradation. In order to determine if there
is more than one enzyme, analogs beyond the scope if this
MALDI-MS was shown to be a good alternative to HPLC based on
neuropeptides and stimulates fat body trehalose synthesis. A
pharmacological HTH concentrations (200 jiM) the primary
phosphoramidon, a specific EP 24.11 inhibitor. HTH
pQ were: PQVNFSPG-OH (HTH 1-7), PQVNFS-OH (HTH 1-5), and
PQVNF-OH (HTH 1-4). HTH 1-7 was present at more than lOx the
PQVNFSPGWGT-NH2) is a member of the AKH/RPCH family of insect
series of Nterminal fragment standards of HTH were used for
study would need to be designed and analyzed. dYl-HTH (Ac-
that this was a major endopeptidase cleavage site. At
the development of an HPLC method for monitoring the
the sensitivity, reproducibility, and reduced analysis time.