Biodegration of hypertrehalosemic hormone in the cockroach Blaberus discoidalis /
and Phe4. This cleavage site was indicative of an
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | and Phe4. This cleavage site was indicative of an and Ser5. it is possible that more than one enzyme might be Blaberus discoidalis hypertrehalosemic hormone (HTH= breakdown products of HTH in hemolymph samples and in vitro concentration of the other two metabolic products indicating degradation product appeared to be a cleavage between Phe4 degradation was also analyzed by matrix-assisted laser desorption and ionization mass spectrometry (MALDI-MS). dYVNFSPGWGT-NH2), an analog of HTH, was cleaved between Asn3 endopeptidase 24.11-like cleavage, but was not inhibited by incubation medium. Examination of [3H-pQl]HTH by HPLC indicated that the breakdown products of HTH relative to the involved in HTH degradation. In order to determine if there is more than one enzyme, analogs beyond the scope if this MALDI-MS was shown to be a good alternative to HPLC based on neuropeptides and stimulates fat body trehalose synthesis. A pharmacological HTH concentrations (200 jiM) the primary phosphoramidon, a specific EP 24.11 inhibitor. HTH pQ were: PQVNFSPG-OH (HTH 1-7), PQVNFS-OH (HTH 1-5), and PQVNF-OH (HTH 1-4). HTH 1-7 was present at more than lOx the PQVNFSPGWGT-NH2) is a member of the AKH/RPCH family of insect series of Nterminal fragment standards of HTH were used for study would need to be designed and analyzed. dYl-HTH (Ac- that this was a major endopeptidase cleavage site. At the development of an HPLC method for monitoring the the sensitivity, reproducibility, and reduced analysis time. |
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| Item Description: | "Major subject: Entomology". Vita. |
| Physical Description: | ix, 58 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |