Detection of chlorpyrifos-ethyl (Dursban) and its metabolites in urine samples using immunoassays with confirmation by gas chromatography/mass spectrometry /

0.08 ppb. It was also selected because it detects the

Bibliographic Details
Main Author: Clewis, Suenda Beth
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1995.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:0.08 ppb. It was also selected because it detects the
85 ppb. While the metabolite's detection limit is not as
attempted to quantify the amount of chlorpyrifos-ethyl in
been developed for measuring chlorpyrifos-ethyl
been sufficient for the purposes of this study. The cELISA
cELISA as a reliable method for detection of chlorpyrifos-
Chlorpyrifos-ethyl (Dursban) belongs to the organophosphate
chromatography/mass spectrometry (GC/MS), and can be
class of pesticides. Many organophosphates, such as
commonly determined by measuring cholinesterase inhibition.
concentrations in water. Compared to conventional
confirmation by GC/MS. The selected cELISA (Millipores
desirable as the parent compounds' detection limit, it is
EnviroGard chlorpyrifos-ethyl plate kit) is a quantitative
ethyl in urine.
ethyl in water samples. Even though the parent compound is
expensive and time consuming. Immunoassay techniques have
for domestic and agricultural purposes because of their
form of Enzyme Linked Immunoabsorbent Assay (cELISA), with
laboratory test traditionally used to detect chlorpyrifos-
metabolized before being excreted in urine, the urinary
methodology, these new techniques are economical, rapid, and
methods. Therefore, it is not yet possible to recommend this
parathion and chlordane, are highly toxic and have restricted
primary metabolite 3,5,6-TCP, which was expected in urine, at
relatively low toxicity. Exposure to chlorpyrifos-ethyl is
samples with the immunoassay. However, traditional GC/MS
samples. No correlation was established between the two
statistically equivalent to GC/MS results. This study
still acceptable. Chlorpyrifos-ethyl was detected in a few
techniques failed to detect this pesticide in the same
This method requires blood. sample analysis via gas
traces of chlorpyrifos-ethyl that are present should have
urine samples using the two-step indirect competitive assay
usage. Others, including chlorpyrifos-ethyl, are widely used
was selected solely because it detects chlorpyrifos-ethyl at
Item Description:"Major subject: Industrial Hygiene".
In title, "Dursban" is lacking symbol for trademark.
Vita.
Physical Description:xi, 84 leaves : illustrations ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.