Hormonal effects on proliferation of adipocyte precursor cells isolated from chicken enbryos /
(GH) , and triiodothyronine (T3) on proliferation of chicken
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (GH) , and triiodothyronine (T3) on proliferation of chicken (P<.Ol), but stimulation wasnot significantly different 103 ng/mL) and bIN (105ng/mL) stimulated cell growth a mixture of bovine and porcine glucagon (MGLU, [ ]31.6 adipocyte precursor cells was examined. Adipocyte precursor adipocyte precursors was proportional to the concentration of aggregates, and stromal-vascular cells were further separated cells were isolated from subcutaneous and f emoral adipose concentrations of bIN did not. Chicken glucagon (cGLU), containing adipocytes in culture. Confuency from initial depots from 18-day embryos using collagenase digestion. The digest was filtered through nylon screens to remove cell FBS added to medium. Cells did not proliferate in serum- free media without hormone treatment. High concentrations of from mature adipocytes by centrifugation. Medium 199 from values achieved with MGLUalone. Addition of T3- hormones did not increase proliferation above values observed Influence of glucagon (GLU), insulin (IN), growth hormone insulin (bIN) (2:10' ng/mL) increased cell sensitivity to T3- multiplication than any examined hormone treatment (P<.Ol). multiplied to conf luency and matured into lipid droplet- ng/mL) and T3 (2:1000 ng/mL) alone significantly (P<.05) of incubation. Multiplication of fibroblastlike cells and plating density ( 104 cel 1S/CM2) was achieved by 6 to 7 days porcine growth hormone (pGH) and bIN alone failed to stimulate cell multiplication. Several combinations of stimulated cell proliferation (P<.Ol) although lower stimulated cell proliferation. High concentrations of bovine supplemented with antibiotics and fetal bovine serum (FBS) to stimulate cell proliferation. Combinations of MGLU (2 X to the effect T3 alone. FBS had a greater impact on cell treated cells to pGH did not increase responsiveness of cells was used to support growth of adipocyte precursors. Cells when a single hormone was used. Porcine GH with bIN failed |
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| Item Description: | "Major subject: Poultry Science". Vita. |
| Physical Description: | ix, 66 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |