Purification of sulfide oxidase from rat liver /
ammonium sulfate at pH 7.2 provided an initial precipitation
| Main Author: | |
|---|---|
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
|
| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | ammonium sulfate at pH 7.2 provided an initial precipitation been isolated and purified by means of ammonium sulfate both are based on measuring substrate disappearance of determine sulfide oxidase activity have been established and electrodes method due to its greater sensitivity and smaller exclusion chromatography. The use of 80% saturation with fractionation, anion-exchange chromatography, and size methods are simple, convenient and accurate. Ultimately the methylene blue method was preferred over the ion selective of sulfide oxidase, provided an initial precipitation of purification of the enzyme was obtained. size sample requirements. Sulfide oxidase from rat liver has sulfide oxidase, and after chromatographic procedures a 21 fold sulfide. Both methylene blue and ion selective electrode The present study represents an initial investigative effort to purify sulfide oxidase from rat liver. Two methods to |
|---|---|
| Item Description: | "Major subject: Medical Sciences". Vita. |
| Physical Description: | viii, 35 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |