Determination of the trophic mode of Beggiatoa spp. found at hydrocarbon seeps on the continental slope of the northern Gulf of Mexico /

and non-pigmented filaments to have rounded ends, cells which

Bibliographic Details
Main Author: Nikolaus, Roxanne Lee
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1995.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:and non-pigmented filaments to have rounded ends, cells which
appear hollow, and other characteristic traits of Beggiatoa.
assays, and DNA extraction, amplification, and probing
C02 by whole, live cells, and RuBisCo assays using cell free
chemoautotrophic capability. Whether they are mixotrophic or
chemoautotrophic, mixotrophic, and heterotrophic.
chemoautotrophic. Pigmented filaments were shown to possess
collected via the slurp mechanism of the DSV Johnson Sea
dependence on organic compounds as a carbon source precludes
did not hybridize with a Pisum sativum probe. The
environment capable of supporting a Beggiatoa population
experiments were used to determine the trophic mode of the
extract, showed non-pigmented filaments to be capable of
filament width class represented, most likely, a single
filamentous, sulfur oxidizing Beggiatoa, are prevalent at
filaments. A non-pigmented sample that contained only one
for energy, categorizes the non-pigmented filaments as
functioning in three distinct trophic modes:
geochemical nature of hydrocarbon seeps creates an
heterotrophic depends on the source of energy used and was
hydrocarbon seeps located on the continental slope of the
hydrothermal vents. Microscopic analysis revealed pigmented
indicated the capacity for H2S oxidation. Incorporation Of
Link. Microscopic analysis was used to determine filament
little C02 incorporation ability. Their consequent
non-pigmented Beggiatoa DNA. However, the non-pigmented DNA
non-pigmented sample and one pigmented sample indicated the
northern Gulf of Mexico. Samples of both filament types were
not clearly determined in this study. PCR using primers from
observed in both pigmented and nonpigmented samples may
Pigmented and non-pigmented bacterial mats, which consist of
possible amplification of the RuBisCo gene in pigmented and
presence of more than one species. Very wide filaments
primary carbon source along with the ability to oxidize H2S
represent a species endemic to hydrocarbon seeps and
significant C02 fixation. This ability to use C02 as the
species. The wide range of filament widths found in a second
Sulfur granules in both pigmented and non-pigmented cells
the large subunit of the RuBisCo gene from Zea showed
width and morphology. C02 incorporation experiments, RuBisCo
Item Description:"Major subject: Oceanography".
Vita.
Physical Description:x, 64 leaves : illustrations, maps ; 28 cm.
Also available online.
Issued also on microfiche from Lange Micrographics.
Bibliography:Includes bibliographical references.