T cell response to oral immunization with cow's milk proteins in guinea pigs /

and 0 caseins. However, reseeding cow's milk to guinea pigs

Bibliographic Details
Main Author: Chitla, Suhasini Reddy, 1967-
Format: Thesis eBook
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1994.
Subjects:
Online Access:Link to OAKTrust copy
Description
Summary:and 0 caseins. However, reseeding cow's milk to guinea pigs
Caseins (cc and 0) and 0-lactoglobulin induced a
caseins (cc and 0) and 0-lactoglobulin, and minimally to cc-
Caseins (cc and 0) and 0lactoglobulin were found to be the
cells. Using flow cytometric analysis, the study revealed a
circulation and mesenteric lymph node which, in turn,
correlated directly with the ability to enhance the
demonstrated for the first time that the oral sensitization
demonstrated that the increased proliferation in nonenfiched
didn't increase the proliferate response, and may even have
guinea pigs fed cow's milk for varying periods of time. Two
guinea pigs. The lymphocyte stimulation assay with highly
in peripheral blood, spleen, and mesenteric lymph node of
induced suppression. Our results suggest that the optimum
lactalbumin and whole milk (p>0.05). Even two months after
length of time required for oral sensitization is 2 weeks.
lymph node and blood. Peripheral blood and mesenteric lymph
lymphoproliferative reaction. Overall, this study
marked difference in the distribution of phenotypic markers
mesenteric lymph node and spleen. On the other hand, 3 weeks
milk allergy in humans.
mitogen (Con A). T cell enrichment prior to culture
ml/day) for a 2-4 week sensitization period. An in vitro
most common sensitizing T cell antigens in orally sensitized
node lymphocytes were quite responsive whereas splenocytes
of sensitization resulted in fewer CD8+ T cells in the
oral sensitization. However, prolonged feeding for 4 weeks
outbred Hartley guinea pigs were fed pasteurized milk (100
proliferated significantly (p<0.05), when cultured with cc
proliferation assay was used to examine the antigen-specific
purified antigens could be useful in the diagnosis of cow's
responded poorly. Peripheral blood lymphocytes from orally
responses in both local and systemic lymphoid compartments.
responses in sensitized lymphocytes from spleen, mesenteric
sensitized guinea pigs responded significantly (p<0.05) to
significantly higher proliferate response after 2 weeks of
spleen, with a predominance of the CD8 subset in the
T cell populations was influenced by antigen presenting
the oral exposure, mesenteric lymph node lymphocytes still
To evaluate the cell-mediated response to oral sensitization,
upregulated the mitogenic response to a polyclonal T cell
weeks of oral sensitization yielded more T lymphocytes in
with cow's milk can induce antigenic-specific T lymphocyte
with cow's milk downregulated the antigenic response and
Item Description:"Major subject: Nutrition".
Vita.
Physical Description:xiii, 85 leaves : illustrations ; 28 cm.
Also available online.
Bibliography:Includes bibliographical references.