Characterization of protein binding to the psbAII promoter region in Synechococcus sp. strain PCC 7942 and its role in light regulated gene expression /
binding as compared to the wild-type promoter fragment. The
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | binding as compared to the wild-type promoter fragment. The binding in DNA mobility shift assays. Reduction in the coli lacz gene in Synechococcus cells grown under low- and conditions, and expression increases with increasing light correlation between the level of protein binding and gene expression. Competition assays with psbAII and other member extracts isolated from Synechococcus cells grown under high- found in the photosystem R reaction center. fragments containing portions of the psbAII promoter region gene is regulated in response to changes in light intensity. have localized DNA sequences required for protein binding to high-light conditions. The promoter fragments used to create high-light intensity, and there appeared to be a direct intensity. DNA mobility shift assays using soluble protein levels of protein binding as a result of deletions within the light conditions indicate that two proteins bind to sequences light-responsive members of these gene families, psbAI, located within the promoter region of the psbAII gene. DNA Low levels of expression are observed under low-light member of a small gene family encoding the DI Protein mobility shift assays and competion assays using small DNA ne PsbAII gene Of Synechococcus Sp. strain PCC 7942 is a of the psba and psbd gene families (encoding the D I and D2 Previous studies have shown that expression of the psbAII promoter fragments to drive the expression of the Escherichia promoter region also reduced the level of gene expression at proteins of photosystem II, respectively) indicated that the psbAII, psbAIII, and psbDII, bind similar proteins and may role of protein binding in light regulated gene expression sequences near these regions reduced the level of protein share a common means of regulation. the regions of +7 to +17 and +25 to +29. Deletion of DNA these reporter constructs showed different levels of protein was examined by testing the ability of psbAII derived |
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| Item Description: | "Major subject: Biology". Vita. |
| Physical Description: | xi, 59 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |