http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Biomarkers of polycyclic aromatic hydrocarbon (PAH) exposure in northwest Gulf of Mexico marine fish and invertebrates
indicators of offshore petroleum contamination
(PAHS) associated with crude oil induce CYPIAI gene
a contaminant gradient. Interestingly, the CYPIAI MRNA data
and EROD activity were detected in all species, though the
and ethoxyresorufin-0-deethylase (EROD) activity (a CYPIAI
at the study sites. All three assays were sensitive
bands did not correlate with EROD activity in the same fish
biomarker of exposure to PAHs in aquatic and marine
differences between the near and far stations as expected for
environments. Several benthic marine fish and invertebrates
exhibited some inter- and intraspecies differences,
expression, and this response has been utilized as a
extracts in rat hepatoma H-4-IIE cells. CYPIAI MRNA levels
hepatoma H-4-IIE cells also indicated low PAH contamination
Higher molecular weight Polycyclic aromatic hydrocarbons
Invertebrate exposure to PAHs was estimated by determining
MRNA levels using a CDNA probe derived from rainbow trout,
or "far", >3000 m). Hepatic tissues were analyzed for CYPIAI
PAH contamination at these sites. Intensities of the MRNA
platforms in the northwest Gulf of Mexico and subdivided with
relative response intensities were low, indicating minimal
respect to distance from the platforms (i.e. "near", <100 m
response) was also determined in the fish species.
species. Bioanalysis of invertebrate extracts in rat
species. The results indicated that there were no consistent
suggesting genetic differences in this gene in various fish
the dose-dependent induction of EROD activity by invertebrate
were collected in the vicinity of offshore petroleum