rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Expression of thioredoxin in Fasciola hepatica
  name:
    name part: Richardson, Charlene Dee
    name part: 1966-
    role:
      role term: creator
  name:
    name part: Doughty, Barbara L.
    role:
      role term: degree committee member.
  name:
    name part: Ficht, Allison R.
    role:
      role term: degree supervisor.
  name:
    name part: Pace, Carlos N.
    role:
      role term: degree committee member.
  name:
    name part: Peterson, David O.
    role:
      role term: degree committee member.
  name:
    name part: Struck, Douglas K.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses.
  origin info:
    place:
      place term: xx
    date issued: 1994
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 118 leaves : illustrations ; 28 cm
  abstract: Fasciola hepatica, known as a common liver fluke is a digenetic trematode which infects a wide variety of mammals including humans and commercially important livestock. The parasite possesses a complex tegument which is composed of proteins produced by developmentally regulated cytons lying beneath a superficial muscle layer. Three types of cytons, designated T0, T1, and T2 are sequentially activated to manufacture proteins throughout the fluke's migration through the mammalian host. These proteins are "shuttled" through cytoplasmic extensions for ultimate incorporation into the tegument and outer glycocalyx. A series of tegument-specific clones designated FH2020.A, FH2020.SL, and FH2020.C were isolated from a $ lambda$gt11 adult F. hepatica cDNA library. Immunohistochemical staining with anti-FH2020 serum confirmed localization of the FH2020 protein to the surface of the tegument covering the spines. Sequence analysis revealed that each of these clones contained a $ sim$500bp region with extensive homology to thioredoxin. Thioredoxin is a ubiquitous 12kD re-dox protein which is highly conserved from prokaryotes to mammals. Western blot data shows the FH2020 clones to encode a 12 kD protein, while northern analysis of total F. hepatica RNA revealed two bands at 530 bp and 1.52 kb which correspond to processed and unprocessed transcripts, respectively. A genomic southern blot indicates that the FH2020 sequence is a single copy gene. Although each of the clones contains coding sequence for thioredoxin, the initial 30bp of the 5$ sp prime$ end of the sequence shows considerable divergence. Ribonuclease protection revealed the presence of a fully protected FH2020.C thioredoxin message and a 1.52 kb transcript distinguished FH2020.C as a pre-processed thioredoxin message. This conclusion is supported by sequence data which indicates a lack of an initiator methionine in frame with the thioredoxin coding sequence as well as the lack of a poly A tail. Genomic PCR construct …
  note: by Charlene Dee Richardson.
  note: "Major subject: Medical Sciences."
  note: Vita.
  note: Ph. D. Texas A & M University 1994
  note: Includes bibliographical references.
  subject:
    topic: Major medical sciences
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-1554836
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-1554836
  record info:
    record content source: TXA
    record creation date: 960605
    record change date: 20220103134810.0
    record identifier: in00001299521
    language of cataloging:
      language term: eng