Role of copper in the regulation of CU, ZN-superoxide dismutase in human K562 erythroleukemia cells and human fibroblasts /
(CuZnSOD) by its copper cofactor was studied in K562
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (CuZnSOD) by its copper cofactor was studied in K562 Activation of the enzyme CU2Zn2-SUperoxide dismutase activation when the copper concentration was 50 [ ] M. At activity, protein, and MRNA are all linked to the cells, copper may activate a preexisting pool of apoSOD as contrast, the addition of zinc had no effect on the basal copper concentration exceeded 10 [ ]M, and reached maximal copper content in Menkes' cells was twice the concentration copper was correlated to the increased CuZnSOD activity. CuZnSOD MRNA in Menkes' fibroblasts was detectable but could erythroleukemia cells and skin fibroblasts. K562 cells were fibroblasts could not be seen by our procedure. These gradually when the cells were incubated with 50 gM CUC12.The incubated in medium supplemented with 0 - 50 IIM CUC12 or intracellular level of copper. level of CuZnSOD activity, even at a concentration of 50 gM. not be quantitated, whereas CuZnSOD MRNA in normal of normal fibroblasts. Menkes' fibroblasts also had about showed that CuZnSOD activity increased when the extracellular slow at first but then accelerated, suggesting that in K562 studies provide supporting evidence that the CuZnSOD the K562 cells by Northern analysis. Both increased this point the activity was about 11.5 times greater than twice the CuZnSOD activity, suggesting that intracellular Two CuZnSOD MRNA species (0.5 and 0.7 kb) were detected in untreated cells. A time study showed that the activation was well as stimulate transcription of CuZnSOD MRNA. In were assayed for CuZnSOD activity. The results of the study ZnCI2 for 24 h and extracts from the cells for each treatment |
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| Item Description: | "Major subject: Biochemistry". In title, numerals are subscript. Vita. |
| Physical Description: | viii, 35 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |