Gene structure and regulation of cryparin, a hydrophobin of the fungus Cryphonectria parasitica /
a disease in chestnut trees called chestnut blight.
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | a disease in chestnut trees called chestnut blight. accumulation over time in liquid grown cultures. Cryparin amino-terminal end that is typical of structural proteins, amplified CDNA product. Putative 5'and 3' regulatory and it has the properties of a lectin (8,19,39). Antibody both EP155/2 and the isogenic strain UEP1, which contains the caused by the presence of a unique double-stranded (ds)RNA. Cryphonectria parasitica is a filamentous fungus that causes dsRNA, to determine steady state levels of cryparin extension of RNA from a dsRNA-free wild type strain EP155/2. Fungal transformations were performed to attempt to delete however, hybridization with labeled cryparin coding sequences hyphae and fruiting bodies and accumulates in large amounts Hypovirulence and decreased sporulation by the fungus are In order to investigate gene function, a gene deletion vector isolated. Structural analysis was conducted by comparison of most highly expressed in log phase of liquid cultures (70). Northern blot analysis was performed on MRNA extracted from on hyphal cell surfaces (14). The gene for cryparin has been One of the regulated host proteins (cryparin) that has been purified has a glycine-serine repeating sequence near the regulation of several host polypeptides and poly(A)+ RNAS. resistance gene confirmed plasmid integration in nuclear DNA, revealed no deletion of the cryparin gene. selected on media containing the antibiotic hygromycin B. sequences were identified as well as intron splicing sites. Southern hybridizations with the labeled hygromycin staining showed that this protein is specific to aerial Symptoms of dsRNA infection are correlated with the down- the cryparin gene. Mitotically stable transformants were the sequence data from the subgenomic DNA clone and PCR The transcription initiation site was identified by primer transcript. SDS-PAGE gels were used to examine protein was constructed using the subgenomic DNA clone of cryparin. was found to be developmentally regulated with MRNA being |
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| Item Description: | "Major subject: Plant Pathology". Vita. |
| Physical Description: | vi, 42 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |