The use of in situ transcription and anti-sense RNA amplification to investigate gene expression in the developing murine embryo /
(NACHR) were developmentally regulated The level of
| Main Author: | |
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | (NACHR) were developmentally regulated The level of (RT/aRNA), new techniques in molecular biology, were used to 8:12. On GD 10:0, 10:1 2, or 12:0, untreated and RA-treated 9:12 and 1 0:0. The expression of RAR-A increased in the among different regions of the neural tube. (2) LM/Bc females and second bronchial arches from each embryo were utilized branchial arch derivatives of RA exposed fetuses. changes may represent fundamental differences in molecular channel gene (Kvl), and the nicotinic receptor subunit al collected from dams sacrificed on gestational day (GD) 9:12 cranial and caudal neural tube regions were observed, these cranially at GD 1 0:0. Although relatively few statistically dams were sacrificed, and the embryos were recovered. First examine developmental expression of a panel of candidate exposure. The application of RT/aRNA techniques have allowed expression for these genes changed significantly between GD expression of the type-1 cellular retinoid binding protein, expression which occurred within specific embryonic for RT/aRNA studies to explore differences in gene expression for the investigation of coordinate changes in gene genes in two separate studies. (1) Cranial and caudal neural In situ transcription and anti-sense RNA amplification neural tube on GD 9:12, while expression of NACHR was higher neural tube with advancing gestational age while Kvl and the nicotinic receptor expression were significantly decreased or 1 0:0. Retinoic acid receptors (RAR-a), the potassium over the same time period. Kvl and the sodium channel gene processes which are responsible for neural tube development significant changes in the expression patterns between Significant differences in the level of expression between structures during development. the CAMP response element-binding protein, NACHR, RAR-Y, and the first and second bronchial arches were observed. The transforming growth factor P2 were increased following RA tube tissue was isolated from LM/Bc embryos, which were were expressed at higher levels in the cranial region of the were treated with 5 mg/kg all-trans retinoic acid (RA) on GD which may lead to congenital malformations in second |
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| Item Description: | "Major subject: Veterinary Anatomy". Vita. |
| Physical Description: | ix, 78 leaves : illustrations ; 28 cm. Also available online. |
| Bibliography: | Includes bibliographical references. |