rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Metabolic engineering of Escherichia Coli central metabolism
    sub title: physiological effects of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase overproduction
  name:
    name part: Chao, Yun-Peng
    name part: 1961-
    role:
      role term: creator
  name:
    name part: Dale, Bruce E.
    role:
      role term: degree committee member.
  name:
    name part: Eubank, Philip T.
    role:
      role term: degree committee member.
  name:
    name part: Liao, James
    role:
      role term: degree supervisor.
  name:
    name part: Wild, James R.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1994
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii, 112 leaves : illustrations ; 28 cm
  abstract: An ultimate goal of metabolic engineering is to achieve the maximum product yield. Much effort to date has been made to remove bottlenecks in the terminal pathways. As a result, the rate-controlling steps have been shifted to central pathways. However, manipulations of the central metabolism may face an inherent resistance, which in turn may result in a significant change in cell physiology and restrict the practical application. To approach a rational design of metabolic pathways, it is important to understand how bacteria respond to alterations in central metabolite pools. In an attempt to investigate this problem, a perturbation in phosphoenolpyruvate (PEP) and oxaloacetate (OAA) levels has been applied. PEP and OAA are precursors for the synthesis of amino acids and key metabolites at the junction between glycolysis and the tricarboxylic acid cycle. The flux between PEP and OAA is mediated by the reactions of PEP carboxylase (Ppc) and PEP carboxykinase (Pck). In this study, we attempted to disturb the flux distribution between PEP and OAA by overexpression of Ppc, Pck, or both. By doing so, we found that (1) overexpression of Ppc reduces glucose consumption but without affecting cell growth, which improves the growth yield on glucose as a result. (2) overexpression of Pck increases glucose uptake rate relative to growth rate. In particular, the expression level of glnA (encoding glutamine synthetase) is under the effects of Pck activity. (3) the growth rate on succinate is not optimized. Increased levels of Pck can increase the growth rate on succinate, indicating the level of Pck in E. coli is suboptimal under this condition. (4) simultaneous expression of Ppc and Pck forms futile cycling which stimulates metabolic activities. In responses to futile cycling, E. coli is forced to raise oxygen and glucose consumption rates. As a consequence, the surplus of glucose uptake results in increased fermentation products.
  note: "Major subject: Chemical Engineernig."
  note: Vita.
  note: Ph. D. Texas A & M University 1994
  note: Includes bibliographical references.
  subject:
    topic: Major chemical engineering
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-1550456
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-1550456
  record info:
    record content source: TXA
    record creation date: 960513
    record change date: 20220103134930.0
    record identifier: in00001298394
    language of cataloging:
      language term: eng