Lipoprotein utilization by bovine granulosa, theca and luteal cells /
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| Other Authors: | , , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1994.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy ProQuest, Abstract |
| Abstract: | Objectives were to determine the effects of high- and low-density lipoproteins (HDL and LDL) and glycerol on steroidogenesis, insulin-like growth factor-I (IGF- 1) production, and viability of 1) granulosa and theca cells harvested from dominant ovulatory (DO) and non-ovulatory (DNO) follicles during the bovine estrous cycle, 2) mixed luteal cells from corpora lutea (CL) collected on Days 4 and 10 of the estrous cycle, and Day 4 luteal cells from CL destined to be short-lived, and 3) small and enriched large luteal cells from CL collected on Days 4 and 10 of the estrous cycle. Both HDL and LDL maintained a higher (p<0.05) numbers of viable granulosa cells at 72 h of culture and increased the number (p< 0.001) of viable theca cells obtained from DO, but not from DNO follicles. Lipoproteins stimulated (p<0.01) proliferation of steroidogenically inactive theca cells, and HDL maximized (p<0.001) progesterone production by both granulosa and theca cells. Low-density lipoproteins plus glycerol attenuated HDL-stimulated progesterone production. Production of IGF-I by granulosa cells was stimulated (p<0.01) by both HDL and LDL, with HDL being more potent (p<0.001). Low- and high-density lipoproteins stimulated (p < 0.003) progesterone production by bovine luteal cells, and in conjunction with LH stimulated (p<0.05) IGF-I production by luteal cells at 144 h. Lipoproteins stimulated (p<0.01) steroidogenically inactive cell proliferation. High-density lipoprotein maintained higher steroidogenically active cell numbers in luteal cells cultured from Days 4 (p<0.05) and 10 (p<0.09) CL, but not from Day 4 short-lived CL. Progesterone production by large luteal cells cultured from Day 4 and 10 CL, as well as small luteal cells (p<0.05) obtained from Day 10 CL, was stimulated (p<0.01) by HDL and LDL. The combination of LDL and HDL maximized (p<0.01) progesterone secretion in small luteal cells cultured from Day 10 CL. Lipoproteins also enhanced (p<0.05) IGF-I production by small and large luteal cells cultured from Day 4 CL. Lipoproteins stimulated (p<0.001) proliferation of steroidogenenically inactive small cells and maintained (p<0.05) 3j3-HSD-positive cells in large and small cells cultured from both Day 4 and 10 CL. In summary, the lipoprotein environment plays an important role in modulating proliferation and steroidogenesis of bovine ovarian cells. |
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| Item Description: | "Major subject: Physiology of Reproduction." Vita. |
| Physical Description: | xvii, 160 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references. |