RFLP-based genetic maps of chromosomes 6A and 6B of tetraploid wheat (Triticum turgidum L.) /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1993.
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| Subjects: | |
| Online Access: | ProQuest, Abstract Link to OAKTrust copy |
| Abstract: | High RFLP frequencies were detected between Triticum turgidum var. durum cv. Langdon and T. t. var. durum cv. Langdon-T. t. var. dicoccoides disomic chromosome 6A and 6B substitution lines. In a test of 32 cDNA, gDNA, and 'known-function' gene probes, the average RFLP frequencies detected using one and five restriction enzymes respectively, were 43% and 78% for 6 A and 40% and 72% for 6B. These Endings indicated that derivatives of the substitution lines, especially, recombinant substitution lines (RSLs) and F2 populations derived by crossing the substitution lines with cv. Langdon, could be efficiently used to construct RFLP-based linkage maps of chromosomes 6A and 6B. RSLs, being homozygous for a pair of recombined chromosomes, are permanent or global mapping populations and have long-term utility. Subsequently, 66 6A RSLs and 50 6A F2 plants and 85 6B RSLs and 31 6B F2 plants were used to develop maps of these chromosomes. Data obtained from study of the RSLs and F2 plants were first analyzed by the Mapmaker computer program to construct two maps for each chromosome, after which the data obtained from the two types of populations were combined to construct one map for each chromosome using the G-Mendel computer program. The linear order of loci is the same on the 6A and 6B maps, except close to the centromere, and all of the 6A and 6B loci, except possibly some 6A loci located near the centromere, are located in the same chromosome arm as are homologous loci in hexaploid wheat Major differences in genetic distances exist, however, between loci in the proximal regions of 6AL and 6BL, the distances being much smaller for the latter than the former. The comparative maps obtained from Mapmaker and G-Mendel are also discussed. |
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| Item Description: | Vita. "Major subject: Genetics." |
| Physical Description: | xi, 75 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references. |