rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: In vitro toxicity of polycyclic aromatic hyrocarbons and halogenated aromatic hydrocarbons to cetacean cells and tissues
  name:
    name part: Carvan, Michael John
    name part: 1964-
    role:
      role term: creator
  name:
    name part: Burghardt, Robert C.
    role:
      role term: degree committee member.
  name:
    name part: Busbee, David L.
    role:
      role term: degree supervisor.
  name:
    name part: Ramos, Kenneth S.
    role:
      role term: degree committee member.
  name:
    name part: Ridgway, Sam H.
    role:
      role term: degree committee member.
  name:
    name part: Tarpley, Raymond J.
    role:
      role term: degree supervisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1993
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: leaves : illustrations ; 28 cm
  abstract: Cetaceans have been found to bioaccumulate high aromatic hydrocarbon tissue residues, and elevated levels of PCB residues in tissues are proposed to have occurred concurrently with recent epizootic deaths of dolphins. The objectives of this study were: (1) to develop and characterize an epithelial cell line derived from dolphin tissues, (2) to investigate the effects of hydrocarbon pollutants on those cells, and (3) to analyze the toxicity of hydrocarbon pollutants on cetacean tissues in vitro. An epithelial cell line, Carvan dolphin kidney (CDK), isolated from a spontaneously aborted female bottlenose dolphin, Tursiops truncatus, grew rapidly with high plating and colony forming efficiencies. These cells were neither transformed nor immortal, displaying contact inhibition, anchorage dependence, the requirement for high concentrations of fetal bovine serum for growth and a stable 2N = 44 karyotype. Velocity sedimentation analysis showed that CDK cells contained nuclear aryl hydrocarbon receptor, suggestive of cytochrome P450 inducibility. CDK cells were exposed in vitro to BaP with/without TCDD and/or the cytochrome P450 inhibitor α-naphthoflavone. BaP inhibited mitosis in CDK cells in a dose-dependent manner. Data indicate that CDK cells metabolize BaP, that BaP metabolites bind to cellular DNA initiating unscheduled DNA synthesis, and that the inhibition of cytochrome P450 metabolism decreased the BaP-associated inhibition of mitosis in dolphin cells. The data also suggest that TCDD acts synergistically to increase the levels of DNA damage by the procarcinogen BaP. Cetacean liver microsomes were isolated and evaluated for the presence of cytochrome P450 proteins by SDS-PAGE, apparent minimum molecular weight determination, and immunoblot analysis. None of the putative P450 proteins hybridized with monoclonal antibodies that recognize other mammalian P450s. P450 activity was induced in cetacean tissue samples and CDK cells by exposure in vitro to one of several cyt…
  note: "Major subject: Veterinary Anatomy and Public Health."
  note: Vita.
  note: Ph. D. Texas A & M University 1993
  note: Includes bibliographical references.
  subject:
    topic: Major veterinary anatomy and public health
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-1523715
  identifier: http://gateway.proquest.com/openurl
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-1523715
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 960322
    record change date: 20220103134922.0
    record identifier: in00001275197
    language of cataloging:
      language term: eng