Isolation of molecular genetic markers and a search for possible associations with parasite resistance and quantitative trait loci in Capra hircus /
| Main Author: | |
|---|---|
| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1993.
|
| Subjects: | |
| Online Access: | ProQuest, Abstract Link to OAKTrust copy |
| Abstract: | Microsatellite markers were isolated by sequencing (GT)n positive pUC19 clones containing size selected (300-900 base pair) goat genomic DNA inserts. After locating (GT)n repeat sequences longer than 18 base pairs, primer sequences were designed and synthesized to amplify the repeat region. Each microsatellite primer pair was included in PCR reactions to amplify genomic DNA from a two generation goat pedigree. This facilitated determination of compliance of the microsatellite marker with Mendelian inheritance. Second, the primer pair was used in similar reactions using DNA templates from 4-way cross goats derived from the four genetically diverse breeds East African, Galla, Anglo Nubian and Toggenburg to determine the degree of polymorphism at the microsatellite locus. Twelve microsatellite markers with polymorphic information content (PIC) values ranging from 0.34 to 0.89 were isolated and scored in 270 goats comprising 21 half-sib families. Sequences for microsatellite markers SR-CRSP-1 to SR-CRSP-10 were submitted to Genbank data library (Accession numbers: L22192, L22194, L22195, L22196, L22197, L22198, L22199, L22193, L22200 and L22201). In addition, three polymorphic RAPD markers were determined to give consistent results and were scored in 53 goats representing 10 half-sib groups. The microsatellite markers SR-CRSP-2 and SR-CRSP-3 were determined to be linked with a lod score of 3.14, a recombination rate of 22% and an estimated distance of 24.2 map units (mu). Linkage of SR-CRSP-2 with SRCRSP-7 at a distance of 30.4 mu was suggested by die data, but the evidence for linkage was not conclusive (lod score = 2.28). The remainder of the microsatellite markers were determined to be unlinked. RAPD marker loci were not linked with each other or with the microsatellite markers. Breedtype, type of birth and gender were significantly associated (P<0.05) with birthweight, weaning weight and yearling weight. Significant associations were also found between microsatellite markers SR-CRSP-7 and birth weight (P<0.04), SR-CRSP-2 and weaning weight (P<0.02), and SR-CRSP-11 and yearling weight (P<0.01). No significant associations were detected between any of the markers and phenotypic measures of parasite resilience. No associations were detected between RAPD markers and any of the dependent variables in this study. |
|---|---|
| Item Description: | Vita. "Major subject: Genetics." |
| Physical Description: | xi, 111 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references. |